巴雷特食管内镜检查有证据的患者杯状细胞和柱状蓝色细胞中细胞角蛋白7和20的免疫反应性。

Immunoreactivity of cytokeratins 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus.

作者信息

Cantarelli João Carlos, Fagundes Renato Borges, Meurer Luise, Rocha Marta Pires da, Nicola André, Kruel Cleber Dario Pinto

机构信息

Postgraduate program: Sciences in Gastroenterology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

出版信息

Arq Gastroenterol. 2009 Apr-Jun;46(2):127-31. doi: 10.1590/s0004-28032009000200010.

DOI:10.1590/s0004-28032009000200010

PMID:19578614

Abstract

CONTEXT

Barrett's esophagus is characterized by the presence of goblet cells. However, when alcian-blue is utilized, another type of cells, called columnar blue cells, is frequently present in the distal esophagus of patients with endoscopic evidence of Barrett's esophagus. Cytokeratin 7 and 20 immunoreactivity has been previously studied in areas of intestinal metaplasia at the esophagogastric junction. However, the expression of these cytokeratins in columnar blue cells has not been characterized.

OBJECTIVE

To compare the expression of cytokeratin 7 and 20 in goblet cells and columnar blue cells in patients with endoscopic evidence of Barrett's esophagus.

METHODS

Biopsies from 86 patients with endoscopic evidence of Barrett's esophagus were evaluated. The biopsies were stained for cytokeratin 7 and 20.

RESULTS

Goblet cells were present in 75 cases and columnar blue cells in 50 cases. Overall, cytokeratin 7 expression was similar in goblet cells and columnar blue cells (P = 0.25), while cytokeratin 20 was more common in goblet cells (P <0.001). In individuals with both cell types, however, cytokeratin 7 staining was the same in goblet and columnar blue cells in 95% of the cases, and cytokeratin 20 staining was the same in 77%.

CONCLUSION

Goblet cells and columnar blue cells have similar immunohistochemical staining patterns for cytokeratins 7 and 20 in patients with endoscopic evidence of Barrett's esophagus.

摘要

背景

巴雷特食管的特征是存在杯状细胞。然而，当使用阿尔辛蓝时，另一种细胞，即柱状蓝细胞，经常出现在有巴雷特食管内镜证据的患者远端食管中。细胞角蛋白7和20的免疫反应性先前已在食管胃交界处的肠化生区域进行过研究。然而，这些细胞角蛋白在柱状蓝细胞中的表达尚未得到描述。

目的

比较有巴雷特食管内镜证据的患者杯状细胞和柱状蓝细胞中细胞角蛋白7和20的表达。

方法

对86例有巴雷特食管内镜证据的患者的活检标本进行评估。对活检标本进行细胞角蛋白7和20染色。

结果

75例存在杯状细胞，50例存在柱状蓝细胞。总体而言，杯状细胞和柱状蓝细胞中细胞角蛋白7的表达相似（P = 0.25），而细胞角蛋白20在杯状细胞中更常见（P <0.001）。然而，在同时存在这两种细胞类型的个体中，95%的病例杯状细胞和柱状蓝细胞中细胞角蛋白7染色相同，77%的病例细胞角蛋白20染色相同。