Lopes César V, Pereira-Lima Júlio, Hartmann Antônio
Departmens of Gastroenterology, Porto Alegre School of Medical Sciences (FFFCMPA)/Santa Casa Hospital, Porto Alegre, Brazil.
Hepatogastroenterology. 2006 Jul-Aug;53(70):511-5.
BACKGROUND/AIMS: Hematoxylin-eosin stain may fail to show intestinal metaplasia in Barrett's esophagus, and PAS-alcian blue may present difficulties of interpretation due to its more heterogeneous staining. We investigated whether Mucin-2 is a good substitute for PAS-alcian blue in the detection of goblet cells.
Biopsy specimens from 47 Barrett's esophagus patients were stained with hematoxylineosin and PAS-alcian blue. Mucin-2 expression was evaluated with monoclonal antibody. The intra- and interobserver agreements about the expression of Mucin-2 and PAS-alcian blue stain were determined by kappa statistics.
PAS-alcian blue and Mucin-2 were positive in all cases. Mucin expression was positive in goblet cells and few columnar cells. PAS-alcian blue showed heterogeneous staining. The columnar epithelium and the submucosal glands were also stained. Intra- and interobserver agreement in the identification of intestinal metaplasia was 100%.
Mucin-2 agreed fully with PAS-alcian blue. The intra- and interobserver agreement was perfect, justifying its use in the diagnosis of Barrett's esophagus.
背景/目的：苏木精-伊红染色可能无法显示巴雷特食管中的肠化生，而过碘酸雪夫-阿尔辛蓝染色由于其染色更不均匀，可能存在解读困难。我们研究了黏蛋白-2在检测杯状细胞方面是否是过碘酸雪夫-阿尔辛蓝的良好替代物。
对47例巴雷特食管患者的活检标本进行苏木精-伊红和过碘酸雪夫-阿尔辛蓝染色。用单克隆抗体评估黏蛋白-2的表达。通过kappa统计确定观察者内和观察者间关于黏蛋白-2和过碘酸雪夫-阿尔辛蓝染色表达的一致性。
所有病例中过碘酸雪夫-阿尔辛蓝和黏蛋白-2均呈阳性。黏蛋白在杯状细胞和少数柱状细胞中表达阳性。过碘酸雪夫-阿尔辛蓝染色不均匀。柱状上皮和黏膜下腺也被染色。观察者内和观察者间在识别肠化生方面的一致性为100%。
黏蛋白-2与过碘酸雪夫-阿尔辛蓝完全一致。观察者内和观察者间的一致性极佳，证明其可用于巴雷特食管的诊断。
