McDuffie R S, Blanton S J, Shikes R H, Gibbs R S
Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Denver.
Am J Obstet Gynecol. 1991 Nov;165(5 Pt 1):1568-74. doi: 10.1016/0002-9378(91)90406-h.
We conducted experiments with a previously described rabbit model of Escherichia coli-induced preterm pregnancy loss. Does at 70% gestation were inoculated hysteroscopically with 0.2 ml of Escherichia coli (10(5) colony-forming units per milliliter) or saline solution. Animals were randomly assigned to either receive treatment with ampicillin-sulbactam (begun 1 to 2 hours before inoculation and continued for up to 7 days) or to receive no therapy. Animals were killed after delivery or after 7 days. Saline solution-inoculated animals had no pregnancy loss. Of the Escherichia coli-inoculated animals, those treated with ampicillin-sulbactam had significantly fewer deliveries, fewer positive cultures, and more live fetuses than the untreated animals (p less than or equal to 0.001). Cultures from multiple sites, amniotic fluid prostaglandin levels, and maternal progesterone levels were obtained, and the placenta, uterus, and fetal lung were histologically evaluated. In the second phase of the study, the Escherichia coli-inoculated animals were treated with ampicillin-sulbactam at one of three times: at inoculation or 2 or 4 hours after inoculation. The Escherichia coli-inoculated does treated with ampicillin-sulbactam at or before inoculation had significantly fewer deliveries, fewer positive cultures, and more live fetuses than the Escherichia coli-inoculated does in which treatment was delayed 4 hours (p less than or equal to 0.01).
我们使用先前描述的大肠杆菌诱导的早产流产兔模型进行了实验。在妊娠70%时，通过宫腔镜向子宫内接种0.2 ml大肠杆菌（每毫升含10(5)个菌落形成单位）或生理盐水。动物被随机分配接受氨苄西林-舒巴坦治疗（在接种前1至2小时开始，持续7天）或不接受治疗。动物在分娩后或7天后处死。接种生理盐水的动物没有发生流产。在接种大肠杆菌的动物中，接受氨苄西林-舒巴坦治疗的动物与未治疗的动物相比，分娩次数明显减少，培养阳性率降低，活胎数量更多（p≤0.001）。获取了多个部位的培养物、羊水前列腺素水平和母体孕酮水平，并对胎盘、子宫和胎儿肺进行了组织学评估。在研究的第二阶段，对接种大肠杆菌的动物在三个时间点之一给予氨苄西林-舒巴坦治疗：接种时、接种后2小时或4小时。与接种后4小时才开始治疗的接种大肠杆菌的母兔相比，在接种时或接种前接受氨苄西林-舒巴坦治疗的接种大肠杆菌的母兔分娩次数明显减少，培养阳性率降低，活胎数量更多（p≤0.01）。
