Dombroski R A, Woodard D S, Harper M J, Gibbs R S
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio.
Am J Obstet Gynecol. 1990 Dec;163(6 Pt 1):1938-43. doi: 10.1016/0002-9378(90)90777-5.
Bacterial infection has been implicated in premature labor in humans. To elucidate mechanisms and potential intervention strategies, we sought to develop a model of infection-induced pregnancy loss in rabbits. On day 21 (70% of gestation), each uterine horn was inoculated hysteroscopically with 0.2 ml containing saline solution of 10(6) cfu Escherichia coli or Bacteroides bivius or Fusobacterium necrophorum. Fetal viability was assessed. Animals were sacrificed at various times or as delivery occurred. Serum progesterone and amniotic fluid prostaglandins were measured. Cultures and histologic sections were prepared. Compared with the saline solution group, E coli and F. necrophorum-inoculated rabbits were significantly more likely to deliver (16 of 16 and six of seven with mean times of 31.9 +/- 10.7 and 28.3 +/- 11.5 hours, respectively for E. coli and F. necrophorum). Positive amniotic fluid cultures for the E. coli group were found in 11 of 12 (92%) and for the F. necrophorum group in three of three cases (100%). Histologic inflammation was seen heavily in both the E. coli and F. necrophorum groups, whereas it was absent in the saline solution group. Inoculation with B. bivius led to a much lower pregnancy loss rate (eight of 32) and less histologic inflammation despite positive uterine cultures in most animals. This model may provide an opportunity to determine mechanisms of clinical or subclinical intraamniotic infection and to test intervention strategies.
细菌感染与人类早产有关。为了阐明其机制和潜在的干预策略，我们试图建立一种兔感染诱导妊娠丢失的模型。在第21天（妊娠期的70%），通过宫腔镜向每个子宫角接种0.2 ml含10(6) cfu大肠杆菌、双路拟杆菌或坏死梭杆菌的盐溶液。评估胎儿的生存能力。在不同时间或分娩时处死动物。测量血清孕酮和羊水前列腺素。制备培养物和组织切片。与盐溶液组相比，接种大肠杆菌和坏死梭杆菌的兔子分娩的可能性显著更高（大肠杆菌组16只中有16只，坏死梭杆菌组7只中有6只，大肠杆菌和坏死梭杆菌的平均分娩时间分别为31.9 +/- 10.7小时和28.3 +/- 11.5小时）。大肠杆菌组12例中有11例（92%）羊水培养阳性，坏死梭杆菌组3例中有3例（100%）阳性。大肠杆菌组和坏死梭杆菌组均可见严重的组织学炎症，而盐溶液组则无。接种双路拟杆菌导致的妊娠丢失率低得多（32只中有8只），尽管大多数动物子宫培养阳性，但组织学炎症较轻。该模型可能为确定临床或亚临床羊膜内感染的机制以及测试干预策略提供机会。
