Ismail A, Hai O K, Kader Z A
Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):301-5. doi: 10.1016/s0006-291x(05)81417-2.
Current studies were undertaken to determine the presence of a specific antigenic protein on the outer membrane of Salmonella typhi. Immunoblot analysis using sera from patients with fevers revealed that the 50 kD band was specifically recognized only by typhoid sera. The 50 kD band located on the outer membrane is protein by nature and is not a Vi (capsular), dH (flagellar), or O9 (somatic) antigen of S. typhi. These results indicate the usefulness of the specific antigen in the development of a serodiagnostic test for typhoid fever since antibodies of both the IgM and IgG class responses were obtained.
开展了当前研究以确定伤寒沙门氏菌外膜上一种特定抗原蛋白的存在。使用发热患者血清进行的免疫印迹分析表明，50 kD条带仅被伤寒血清特异性识别。位于外膜上的50 kD条带本质上是蛋白质，不是伤寒沙门氏菌的Vi（荚膜）、dH（鞭毛）或O9（菌体）抗原。这些结果表明该特异性抗原在开发伤寒热血清诊断试验中的有用性，因为获得了IgM和IgG类反应的抗体。
