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使用伤寒血清对伤寒杆菌脂多糖(LPS)进行免疫印迹分析。

Immunoblot analysis of Salmonella typhi lipopolysaccharide (LPS) using typhoid sera.

作者信息

Vullo V, Mastroianni C M, Contini C, Cignarella L, Massetti A P, Falciano M, Paoletti F, Di Seri M

机构信息

Istituto di Malattie Infettive dell'Università La Sapienza di Roma, III Cattedra di Malattie Infettive, Policlinico Umberto I, Italy.

出版信息

Boll Ist Sieroter Milan. 1987;66(2):130-3.

PMID:2444235
Abstract

Lipopolysaccharide (LPS) of Salmonella typhi has been analyzed by immunoblotting with pooled sera from typhoid patients. Pooled typhoid sera have recognized all the antigenic determinants of S. typhi LPS, giving a strong reaction with the repeating units on the O-side chains as well as with the core region. Cross-reacting antigens have been observed with the LPS of S. typhimurium and S. enteritidis, while no heterologous reactions were seen with the LPS of E. coli strains.

摘要

利用伤寒患者的混合血清通过免疫印迹法对伤寒沙门氏菌的脂多糖(LPS)进行了分析。混合伤寒血清识别出了伤寒沙门氏菌LPS的所有抗原决定簇,与O侧链上的重复单元以及核心区域产生了强烈反应。已观察到与鼠伤寒沙门氏菌和肠炎沙门氏菌的LPS存在交叉反应抗原,而与大肠杆菌菌株的LPS未观察到异源反应。

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