Pandey V N, Dave V P, Amrute S B, Rosenbach K, Modak M J
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):95-9. doi: 10.1016/s0006-291x(05)81386-5.
It was previously reported that nuclear matrix isolated from young rat thymus contained an activity that supported V(D)J recombination at a high efficiency (Dave et al., BIOCHEMISTRY 30: 4763-4767, 1991). A similar type of activity is also detected in the matrix prepared from fetal calf thymus. However, restriction enzyme mapping analyses of the recombined product clearly suggest that the double antibiotic resistance exhibited by the matrix treated plasmid substrate is not a consequence of V(D)J signal sequence recombination.
先前有报道称，从幼鼠胸腺中分离出的核基质含有一种能高效支持V(D)J重组的活性（Dave等人，《生物化学》30: 4763 - 4767，1991）。在从胎牛胸腺制备的基质中也检测到了类似类型的活性。然而，对重组产物的限制性酶切图谱分析清楚地表明，经基质处理的质粒底物所表现出的双重抗生素抗性并非V(D)J信号序列重组的结果。
