Pandey V N, Dave V P, Patil M S, Pradhan D S, Amrute S B, Modak M J
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Biochemistry. 1990 May 1;29(17):4037-41. doi: 10.1021/bi00469a002.
Nuclear matrix prepared from 2-3 week old rat thymuses contains tightly bound TdT activity which has been quantitatively solubilized with nonionic detergent and sonication. TdT is contained in a discrete complex with a sedimentation value of 23 S. The complex is retained on an anti-TdT antibody column and contains DNA ligase and 3'-5' exonuclease activities as well as DNA and several other proteins but is devoid of replicative DNA polymerases. Such a type of multienzyme complex is absent from the nuclear extracts of thymus prepared from older rats and also from liver and spleen extracts of young and old rats.
从2 - 3周龄大鼠胸腺制备的核基质含有紧密结合的末端脱氧核苷酸转移酶（TdT）活性，该活性已通过非离子去污剂和超声处理定量溶解。TdT存在于一种沉降值为23 S的离散复合物中。该复合物保留在抗TdT抗体柱上，含有DNA连接酶和3'-5'核酸外切酶活性以及DNA和其他几种蛋白质，但缺乏复制性DNA聚合酶。这种多酶复合物在老年大鼠制备的胸腺核提取物以及年轻和老年大鼠的肝脏和脾脏提取物中均不存在。
