Bobowiec Ryszard, Wessely-Szponder Joanna, Hola Piotr
University of Life Sciences, Department of Pathophysiology, Chair of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, Akademicka 12 20-033 Lublin, Poland.
Acta Vet Hung. 2009 Jun;57(2):283-93. doi: 10.1556/AVet.57.2009.2.9.
Coagulation and inflammation are closely related as part of the mechanisms of host defence during a severe infection. The aim of this study was to investigate the relation between thrombin as a factor in both the coagulative and inflammatory processes and neutrophil secretory function on the basis of lactoferrin (LF), elastase and myeloperoxidase release in the course of mastitis and metritis in cows. Thrombin generation was measured on the basis of hydrolysis of SAR-PRO-ARG-pNA and lactoferrin concentration was estimated by an ELISA method. The greatest thrombin generation was observed in the metritis group (1.18 +/- 0.62 IU). The level of LF was the highest in the group of cows with mastitis (0.74 +/- 0.55 mg/ml) in the first phase of the disease. In the second phase of the diseases the level of serum LF in cows with mastitis diminished to the value of 0.41 +/- 0.16 mg/ml, whereas in cows with metritis the level of LF increased to 0.51 +/- 0.17 mg/ml. This study reveals that the excessive production of thrombin not only causes hypercoagulatory disorders but also exaggerates neutrophil function by the release of some enzymes which may play a destructive role during disseminated intravascular coagulation (DIC). These enzymes also inhibit anticoagulative systems, thus potentially worsening the course of the disease.
在严重感染期间，凝血和炎症作为宿主防御机制的一部分密切相关。本研究的目的是基于奶牛乳腺炎和子宫炎病程中乳铁蛋白（LF）、弹性蛋白酶和髓过氧化物酶的释放，研究凝血和炎症过程中的凝血酶与中性粒细胞分泌功能之间的关系。基于SAR-PRO-ARG-pNA的水解来测定凝血酶生成，并用ELISA方法估算乳铁蛋白浓度。在子宫炎组中观察到最大的凝血酶生成（1.18±0.62 IU）。在疾病的第一阶段，乳腺炎奶牛组的LF水平最高（0.74±0.55 mg/ml）。在疾病的第二阶段，乳腺炎奶牛的血清LF水平降至0.41±0.16 mg/ml，而子宫炎奶牛的LF水平升至0.51±0.17 mg/ml。本研究表明，凝血酶的过度产生不仅会导致高凝紊乱，还会通过释放一些酶来夸大中性粒细胞功能，这些酶在弥散性血管内凝血（DIC）期间可能起破坏作用。这些酶还会抑制抗凝系统，从而可能使疾病进程恶化。
