Department of Pediatrics, Taipei Medical University Hospital, Taipei, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:408714. doi: 10.1093/ecam/nep073. Epub 2011 Jan 4.
Baicalin is a flavonoid compound purified from the medicinal plant Scutellaria baicalensis Georgi and has been reported to stimulate surfactant protein (SP)-A gene expression in human lung epithelial cell lines (H441). The aims of this study were to determine whether maternal baicalin treatment could increase lung surfactant production and induce lung maturation in fetal rats. This study was performed with timed pregnant Sprague-Dawley rats. One-day baicalin group mothers were injected intraperitoneally with baicalin (5 mg/kg/day) on Day 18 of gestation. Two-day baicalin group mothers were injected intraperitoneally with baicalin (5 mg/kg/day) on Days 17 and 18 of gestation. Control group mothers were injected with vehicle alone on Day 18 of gestation. On Day 19 of gestation, fetuses were delivered by cesarean section. Maternal treatment with 2-day baicalin significantly increased saturated phospholipid when compared with control group and total phospholipid in fetal lung tissue when compared with control and 1-day baicalin groups. Antenatal treatment with 2-day baicalin significantly increased maternal growth hormone when compared with control group. Fetal lung SP-A mRNA expression and maternal serum corticosterone levels were comparable among the three experimental groups. Maternal baicalin treatment increases pulmonary surfactant phospholipids of fetal rat lungs and the improvement was associated with increased maternal serum growth hormone. These results suggest that antenatal baicalin treatment might accelerate fetal rat lung maturation.
黄芩苷是从药用植物黄芩中提取的一种黄酮类化合物，已被报道能刺激人肺上皮细胞系（H441）中表面活性蛋白（SP）-A 基因的表达。本研究旨在确定母体黄芩苷治疗是否能增加肺表面活性物质的产生并诱导胎儿肺成熟。本研究采用定时妊娠 Sprague-Dawley 大鼠进行。1 天黄芩苷组母鼠于妊娠第 18 天腹腔内注射黄芩苷（5mg/kg/天）。2 天黄芩苷组母鼠于妊娠第 17 天和第 18 天腹腔内注射黄芩苷（5mg/kg/天）。对照组母鼠于妊娠第 18 天腹腔内注射溶剂。妊娠第 19 天，通过剖宫产娩出胎儿。与对照组相比，2 天黄芩苷处理组胎鼠肺组织中饱和磷脂显著增加，与对照组和 1 天黄芩苷组相比，总磷脂也显著增加。与对照组相比，产前 2 天黄芩苷处理组母鼠生长激素显著增加。三组实验中，胎鼠肺 SP-A mRNA 表达和母鼠血清皮质酮水平无差异。母体黄芩苷处理增加了胎鼠肺的肺表面活性物质磷脂，改善与母血清生长激素增加有关。这些结果表明，产前黄芩苷处理可能加速胎鼠肺成熟。
