Khosla S S, Rooney S A
Am J Obstet Gynecol. 1979 Jan 15;133(2):213-6. doi: 10.1016/0002-9378(79)90479-4.
We have investigated the effect of 17beta-estradiol on lung surfactant production in the fetal rabbit. Pregnant rabbits were administered intramuscularly 75microgram of 17beta-estradiol per day at 25 and 26 days' gestation. Control rabbits were similarly injected with the solvent only. At 27 days' gestation the fetuses were delivered by cesarean section and the lungs of the newborn rabbits were lavaged in situ with 0.9 per cent NaCl. Analysis of lung lavage and lavaged lung tissue phospholipids yielded the following results: (a) The 17beta-estradiol--treated group had three to four times more total phospholipid and phosphatidylcholine in the lung lavage than the control group. (b) The phosphatidylcholine/sphingomyelin ratio in the lung lavage was significantly higher in the treated than in the control group. (c) The lavaged lung tissue of the treated group had almost twice as much total phospholipid and phosphatidylcholine as the control group. These changes in lung phospholipid content and composition are in the direction of increased lung maturity and suggest that estrogen may have a role in fetal lung maturation and surfactant production.
我们研究了17β-雌二醇对胎兔肺表面活性物质产生的影响。在妊娠25天和26天时，给怀孕的兔子每天肌肉注射75微克17β-雌二醇。对照兔同样仅注射溶剂。在妊娠27天时，通过剖宫产取出胎儿，并用0.9%的氯化钠对新生兔的肺进行原位灌洗。对肺灌洗物和灌洗后的肺组织磷脂进行分析，得出以下结果：(a) 17β-雌二醇处理组肺灌洗物中的总磷脂和磷脂酰胆碱比对照组多三到四倍。(b) 处理组肺灌洗物中磷脂酰胆碱/鞘磷脂的比值显著高于对照组。(c) 处理组灌洗后的肺组织中总磷脂和磷脂酰胆碱几乎是对照组的两倍。肺磷脂含量和组成的这些变化表明肺成熟度增加，提示雌激素可能在胎儿肺成熟和表面活性物质产生中起作用。
