Adams Lynn S, Seeram Navindra P, Hardy Mary L, Carpenter Catherine, Heber David
Center for Human Nutrition, David Geffen School of Medicine, University of California at Los Angeles, 90095-1742, USA.
Evid Based Complement Alternat Med. 2006 Mar;3(1):117-24. doi: 10.1093/ecam/nel001.
Herbal medicines are often combinations of botanical extracts that are assumed to have additive or synergistic effects. The purpose of this investigation was to compare the effect of individual botanical extracts with combinations of extracts on prostate cell viability. We then modeled the interactions between botanical extracts in combination isobolographically. Scutellaria baicalensis, Rabdosia rubescens, Panax-pseudo ginseng, Dendranthema morifolium, Glycyrrhiza uralensis and Serenoa repens were collected, taxonomically identified and extracts prepared. Effects of the extracts on cell viability were quantitated in prostate cell lines using a luminescent ATP cell viability assay. Combinations of two botanical extracts of the four most active extracts were tested in the 22Rv1 cell line and their interactions assessed using isobolographic analysis. Each extract significantly inhibited the proliferation of prostate cell lines in a time- and dose-dependent manner except S. repens. The most active extracts, S. baicalensis, D. morifolium, G. uralensis and R. rubescens were tested as two-extract combinations. S. baicalensis and D. morifolium when combined were additive with a trend toward synergy, whereas D. morifolium and R. rubescens together were additive. The remaining two-extract combinations showed antagonism. The four extracts together were significantly more effective than the two-by-two combinations and the individual extracts alone. Combining the four herbal extracts significantly enhanced their activity in the cell lines tested compared with extracts alone. The less predictable nature of the two-way combinations suggests a need for careful characterization of the effects of each individual herb based on their intended use.
草药通常是植物提取物的组合，假定具有相加或协同作用。本研究的目的是比较单一植物提取物与提取物组合对前列腺细胞活力的影响。然后，我们用等效线图法模拟了植物提取物组合之间的相互作用。采集了黄芩、冬凌草、三七、菊花、甘草和锯叶棕，进行了分类鉴定并制备了提取物。使用发光ATP细胞活力测定法对前列腺细胞系中提取物对细胞活力的影响进行了定量分析。在22Rv1细胞系中测试了四种活性最高的提取物中两种植物提取物的组合，并使用等效线图分析评估了它们之间的相互作用。除锯叶棕外，每种提取物均以时间和剂量依赖性方式显著抑制前列腺细胞系的增殖。测试了活性最高的提取物黄芩、菊花、甘草和冬凌草的两种提取物组合。黄芩和菊花组合时具有相加作用且有协同趋势，而菊花和冬凌草组合时具有相加作用。其余两种提取物组合表现出拮抗作用。四种提取物一起使用比两两组合和单独使用单一提取物的效果显著更好。与单独的提取物相比，四种草药提取物组合在测试的细胞系中显著增强了它们的活性。双向组合的不可预测性更强，这表明需要根据每种草药的预期用途仔细表征其效果。
