Takakura Y, Audus K L, Borchardt R T
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
Adv Pharmacol. 1991;22:137-65. doi: 10.1016/s1054-3589(08)60034-4.
The development of in vitro BBB models consisting of isolated brain capillaries and cultured brain microvessel endothelial cells has made possible the study of BBB transport phenomena at the cellular level. Basic characteristics of BBB transport of endogenous and exogenous solutes and their biochemical, pharmacological, ontogenic, and pathological regulation mechanisms have been investigated. This information has led not only to a better understanding of BBB transport but also to the construction of strategies for improving drug delivery to the CNS for diagnosis and therapeutics. To elucidate the complexity of BBB transport, in vivo studies are always necessary at some point; however, in vitro systems can be useful complements to the in vivo systems. The tissue culture systems seem to be especially important in the clarification of cellular, biochemical and molecular features of BBB transport. Appropriate systems should be selected or combined, depending on the purpose of the investigation.
由分离的脑毛细血管和培养的脑微血管内皮细胞组成的体外血脑屏障模型的发展，使得在细胞水平上研究血脑屏障转运现象成为可能。已经对内源性和外源性溶质的血脑屏障转运的基本特征及其生化、药理、个体发生和病理调节机制进行了研究。这些信息不仅有助于更好地理解血脑屏障转运，还有助于构建改善药物向中枢神经系统递送以用于诊断和治疗的策略。为了阐明血脑屏障转运的复杂性，在某些时候体内研究总是必要的；然而，体外系统可以作为体内系统的有用补充。组织培养系统在阐明血脑屏障转运的细胞、生化和分子特征方面似乎尤为重要。应根据研究目的选择或组合合适的系统。
