[激光捕获显微切割纯化鼻咽癌组织蛋白质表达谱的建立]
[Establishment of protein expression profile of laser capture microdissection-purified nasopharyngeal carcinoma tissue].
作者信息
Peng Fang, Tang Can'e, Li Maoyu, Li Cui, Chen Ailan, Li Feng, Zhang Pengfei, Li Meixiang, Xiao Zhiqiang, Chen Zhuchu
机构信息
Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha 410008, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009 Jun;34(6):481-6.
OBJECTIVE
To establish the protein expression map of nasopharyngeal carcinoma (NPC), and provide a basis for proteomic study of NPC.
METHODS
Laser capture microdissection (LCM) was used to isolate cancer cells from NPC tissues. Two-dimensional gel electrophoresis(2-DE) was used to separate the total proteins of LCM purified NPC cells. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) was performed to identify the proteins, and bioinformatics was used to construct the 2-DE database of NPC proteome.
RESULTS
A 2-DE reference map of NPC was established. On the 2-DE map, a total of (1 312+/-30) protein spots were detected, and 427 protein spots representing 241 non-redundant proteins were identified. The 2-DE database of NPC proteome was constructed. These data could be accessed at our website (http://www.xyproteomics.org).
CONCLUSION
A protein expression profile of LCM purified NPC tissues has been established for the first time, which provides useful information and source for proteomic study of NPC.
目的
建立鼻咽癌（NPC）蛋白质表达图谱，为NPC的蛋白质组学研究提供依据。
方法
采用激光捕获显微切割（LCM）技术从NPC组织中分离癌细胞。用双向凝胶电泳（2-DE）分离LCM纯化的NPC细胞总蛋白。采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定蛋白质，并用生物信息学构建NPC蛋白质组的2-DE数据库。
结果
建立了NPC的2-DE参考图谱。在2-DE图谱上，共检测到（1 312±30）个蛋白点，鉴定出代表241种非冗余蛋白的427个蛋白点。构建了NPC蛋白质组的2-DE数据库。这些数据可在我们的网站（http://www.xyproteomics.org）上获取。
结论
首次建立了LCM纯化的NPC组织的蛋白质表达谱，为NPC的蛋白质组学研究提供了有用信息和资源。
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