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大肠杆菌依赖DNA的RNA聚合酶在不同来源DNA模板上的活性。环磷酸腺苷(cAMP)的作用。

The activity of Escherichia coli DNA-dependent RNA polymerase on DNA templates of different origin. The effect of cAMP.

作者信息

Jiresová M, Janecek J

出版信息

Folia Microbiol (Praha). 1977;22(3):168-72. doi: 10.1007/BF02885597.

Abstract

DNA isolated from different T phages served as a better template for the synthetic activity of unmodified Escherichia coli RNA polymerase in the in vitro system than did the host DNA. cAMP significantly stimulated the activity of such a preparation of RNA polymerase. The stimulation was more pronounced with the host DNA template than with phage DNA. However, the synthetic activity of Escherichia coli RNA polymerase was greater in the presence of cAMP than without it when phage DNA served as the template.

摘要

与宿主DNA相比,从不同T噬菌体中分离出的DNA在体外系统中是未修饰的大肠杆菌RNA聚合酶合成活性的更好模板。环磷酸腺苷(cAMP)显著刺激了这种RNA聚合酶制剂的活性。与噬菌体DNA模板相比,宿主DNA模板的刺激作用更明显。然而,当以噬菌体DNA为模板时,存在cAMP时大肠杆菌RNA聚合酶的合成活性比不存在时更高。

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