Oleson A E, Pispa J P, Buchanan J M
Proc Natl Acad Sci U S A. 1969 Jun;63(2):473-80. doi: 10.1073/pnas.63.2.473.
Sköld and Buchanan(1) have reported that there is a rapid loss of RNA polymerase activity in Escherichia coli B after infection with T4 bacteriophage. More recent studies on the mechanism of this inactivation have been made in this(2) and other laboratories.(3, 4) In this communication, we report the observation of a transient stimulation of RNA polymerase activity when measurement is made immediately after infection and when cells are ruptured by a gentle lysis procedure. The increase in activity is independent of the synthesis of protein. The activity in the extracts of infected cells is lost by treatment of the extract with antibody to E. coli RNA polymerase and is refractive to the inhibitory action of the antibiotic rifamycin. Hybridization experiments indicate that an RNA transcribed almost exclusively from a T4 DNA template is the product of incubation of extracts of infected cells with a reaction mixture containing an exogenous primer (salmon sperm DNA). These findings are consistent with the hypothesis that one of the first steps in phage infection is the formation of a transcription complex containing T4 DNA and E. coli RNA polymerase.
斯科尔德和布坎南（1）报道，用T4噬菌体感染大肠杆菌B后，其RNA聚合酶活性迅速丧失。最近，本实验室（2）及其他实验室（3, 4）对这种失活机制进行了更多研究。在本报告中，我们报道了一个观察结果：当在感染后立即进行测量且细胞通过温和的裂解程序破裂时，会观察到RNA聚合酶活性的短暂刺激。活性的增加与蛋白质合成无关。用抗大肠杆菌RNA聚合酶抗体处理感染细胞提取物后，提取物中的活性丧失，且对抗生素利福平的抑制作用具有抗性。杂交实验表明，几乎完全从T4 DNA模板转录而来的RNA是感染细胞提取物与含有外源引物（鲑鱼精DNA）的反应混合物孵育的产物。这些发现与噬菌体感染的第一步是形成包含T4 DNA和大肠杆菌RNA聚合酶的转录复合物这一假设一致。
