Schlingmann Tobias R, Rininsland Frauke H, Bartholomae Wolf C, Kuekrek Haydar, Lehmann Paul V, Tary-Lehmann Magdalena
Department of Pathology, Case Western Reserve University, Cleveland, OH, USA.
Clin Immunol. 2009 Oct;133(1):45-51. doi: 10.1016/j.clim.2009.06.004. Epub 2009 Jul 9.
EMT-6 mammary carcinoma and B16 melanoma (B16M) cells are lethal and barely immunogenic in syngeneic BALB/c and C57BL/6 mice, respectively. We show that mice vaccinated with tumor cells pulsed with a MHC class I-restricted peptide develop a T cell response, not only to the peptide, but also to the unpulsed tumor. These mice display protective immunity against the unpulsed tumor, and their T cells adoptively transfer tumor-specific protection to immunodeficient SCID mice. Our data have implications for cancer vaccine strategies. Grafting a single well-defined foreign peptide on tumor cells might suffice to trigger anti-tumor immunity.
EMT-6乳腺癌细胞和B16黑色素瘤(B16M)细胞分别在同基因的BALB/c和C57BL/6小鼠中具有致死性且几乎没有免疫原性。我们发现,用与主要组织相容性复合体I类(MHC I类)限制性肽脉冲处理过的肿瘤细胞进行疫苗接种的小鼠,不仅会产生针对该肽的T细胞反应,还会产生针对未脉冲处理的肿瘤的T细胞反应。这些小鼠对未脉冲处理的肿瘤表现出保护性免疫,并且它们的T细胞能将肿瘤特异性保护作用过继转移给免疫缺陷的严重联合免疫缺陷(SCID)小鼠。我们的数据对癌症疫苗策略具有启示意义。在肿瘤细胞上嫁接单一明确的外源肽可能足以触发抗肿瘤免疫。