Lai P K, Alpers M P, MacKay-Scollay E M
Infect Immun. 1977 Jul;17(1):28-35. doi: 10.1128/iai.17.1.28-35.1977.
Eight patients with infectious mononucleosis, aged between 8 and 24, were studied for cell-mediated immunity by the in vitro leukocyte migration inhibition test at acute and convalescent stages. Follow-up studies were also carried out at up to 4 months after clinical illness. Cell-mediated immunity to Epstein-Barr virus (EBV) in the peripheral leukocytes from these patients was absent or incipient in all cases during the acute phase, although it was present in lymphocytes from a biopsied lymph node obtained from one of the patients. In contrast, cell-mediated immunity to EBV was detected readily in peripheral leukocytes obtained during convalescence and in the follow-up studies. A blocking factor that abrogated leukocyte migration inhibition induced by EBV antigen was detected in acute and convalescent sera obtained from six of eight patients, whereas serum antinuclear autoantibodies were detected in the two patients whose sera failed to block leukocyte migration inhibition. When sera were fractionated, this blocking effect was observed only in the serum immunoglobulin G fractions. In follow-up studies, neither the blocking factor nor the antinuclear autoantibodies were found in the sera collected.
对8例年龄在8至24岁之间的传染性单核细胞增多症患者，在急性期和恢复期通过体外白细胞游走抑制试验研究其细胞介导免疫。在临床发病后长达4个月时也进行了随访研究。这些患者急性期外周血白细胞中对EB病毒（EBV）的细胞介导免疫在所有病例中均不存在或刚刚开始出现，尽管从其中1例患者获取的活检淋巴结的淋巴细胞中存在这种免疫。相比之下，在恢复期获取的外周血白细胞以及随访研究中很容易检测到对EBV的细胞介导免疫。在8例患者中6例患者急性期和恢复期血清中检测到一种能消除EBV抗原诱导的白细胞游走抑制的阻断因子，而在其血清未能阻断白细胞游走抑制的2例患者中检测到血清抗核自身抗体。当血清进行分级分离时，这种阻断作用仅在血清免疫球蛋白G级分中观察到。在随访研究中，所收集的血清中未发现阻断因子和抗核自身抗体。
