Wainwright W H, Veltri R W, Sprinkle P M
J Infect Dis. 1979 Jul;140(1):22-32. doi: 10.1093/infdis/140.1.22.
Lymphocytes from 80% of patients with infectious mononucleosis in this study failed to produce macrophage migration-inhibition factor in response to partially purified early antigen of Epstein-Barr virus or to tetanus toxoid, whereas lymphocytes from normal subjects did produce this lymphokine. Subsequent analysis of serum from the patients with infectious mononucleosis revealed a serum factor that completely abrogated antigen-specific inhibition of migration by human leukocytes as well as lymphocyte blastogenesis. The serum blocking factor was present in sera from 11 (73%) of 15 patients with infectious mononucleos but only in sera from two (13%) of 15 normal subjects. Samples of serum from five of the patients with infectious mononucleosis and five normal subjects were fractionated with use of Sephadex G-200 gel filtration, and the eluants were assayed for several substances known to inhibit cell-mediated immunity. Serum blocking factor activity could be demonstrated only in fractionated sera from patients with infectious mononucleosis. The serum blocking factor is postulated to be either a soluble immune complex or some as yet unidentified immunoregulatory globulin contained in the IgG fraction of human serum.
在本研究中，80%的传染性单核细胞增多症患者的淋巴细胞在受到部分纯化的爱泼斯坦-巴尔病毒早期抗原或破伤风类毒素刺激时，无法产生巨噬细胞移动抑制因子，而正常受试者的淋巴细胞则能产生这种淋巴因子。随后对传染性单核细胞增多症患者的血清进行分析，发现一种血清因子，它能完全消除人类白细胞对迁移的抗原特异性抑制以及淋巴细胞增殖。15例传染性单核细胞增多症患者中有11例（73%）的血清中存在这种血清阻断因子，而15名正常受试者中只有2例（13%）的血清中有该因子。对5例传染性单核细胞增多症患者和5名正常受试者的血清样本进行葡聚糖G-200凝胶过滤分级分离，并对洗脱液中几种已知可抑制细胞介导免疫的物质进行检测。血清阻断因子活性仅在传染性单核细胞增多症患者的分级分离血清中得到证实。推测血清阻断因子要么是一种可溶性免疫复合物，要么是人类血清IgG部分中某种尚未鉴定的免疫调节球蛋白。
