Miura Kyoko, Okada Yohei, Aoi Takashi, Okada Aki, Takahashi Kazutoshi, Okita Keisuke, Nakagawa Masato, Koyanagi Michiyo, Tanabe Koji, Ohnuki Mari, Ogawa Daisuke, Ikeda Eiji, Okano Hideyuki, Yamanaka Shinya
Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.
Nat Biotechnol. 2009 Aug;27(8):743-5. doi: 10.1038/nbt.1554. Epub 2009 Jul 9.
We evaluated the teratoma-forming propensity of secondary neurospheres (SNS) generated from 36 mouse induced pluripotent stem (iPS) cell lines derived in 11 different ways. Teratoma-formation of SNS from embryonic fibroblast-derived iPS cells was similar to that of SNS from embryonic stem (ES) cells. In contrast, SNS from iPS cells derived from different adult tissues varied substantially in their teratoma-forming propensity, which correlated with the persistence of undifferentiated cells.
我们评估了由11种不同方式获得的36个小鼠诱导多能干细胞（iPS）系所产生的次级神经球（SNS）形成畸胎瘤的倾向。来自胚胎成纤维细胞衍生的iPS细胞的SNS形成畸胎瘤的情况与来自胚胎干细胞（ES）的SNS相似。相比之下，来自不同成年组织的iPS细胞的SNS在形成畸胎瘤的倾向上有很大差异，这与未分化细胞的持续存在相关。
