Institute of Nutrition and Health Science, Chia-Nan University of Pharmacy and Science, Tainan 71710, Taiwan.
J Clin Biochem Nutr. 2009 Jul;45(1):20-8. doi: 10.3164/jcbn08-250. Epub 2009 Jun 30.
An oxidized frying oil (OFO) diet has been reported to induce an increase in lipid peroxidation and a reduction in vitamin E status in animal tissues. This study was performed to investigate how vitamin E metabolism is influenced by OFO. Male Wistar rats were divided into three groups, a control group (CO) and two OFO-fed groups (OF and OFE). The diet of the OFE group was supplemented with an extra 50 mg/kg of alpha-tocopherol acetate and thus contained twice as much vitamin E as that of the OF group. After six weeks on these diets, liver alpha-tocopherol levels in the OF group were the significantly lowest among the three groups. Excretion of the alpha-tocopherol metabolite, alpha-carboxyethyl hydroxychroman (alpha-CEHC) in the urine was significantly lower in the OF group than in the other two groups. There were no significant differences in protein levels of alpha-tocopherol transfer protein (alpha-TTP) and multidrug resistance protein among the three groups. Protein levels of cytochrome P450 monooxygenase (CYP) 3A, CYP4A, and catalase were markedly increased in both groups on the OFO diet. This suggests that an OFO diet may interfere with medicine metabolism and needs further investigation.
氧化食用油（OFO）饮食已被报道会导致动物组织中的脂质过氧化增加和维生素 E 状态减少。本研究旨在探讨 OFO 如何影响维生素 E 代谢。雄性 Wistar 大鼠分为三组，对照组（CO）和两组 OFO 喂养组（OF 和 OFE）。OFE 组的饮食补充了额外的 50mg/kg 的α-生育酚醋酸酯，因此其维生素 E 含量是 OF 组的两倍。在这些饮食六周后，OF 组的肝脏α-生育酚水平在三组中最低。尿液中α-生育酚代谢物α-羧乙基羟色满（α-CEHC）的排泄在 OF 组明显低于其他两组。三组之间α-生育酚转移蛋白（α-TTP）和多药耐药蛋白的蛋白水平没有显著差异。OFO 饮食组的细胞色素 P450 单加氧酶（CYP）3A、CYP4A 和过氧化氢酶的蛋白水平明显升高。这表明 OFO 饮食可能会干扰药物代谢，需要进一步研究。
