Liu J F, Lee Y W
School of Nutrition and Health Science, Taipei Medical College, Taipei, Taiwan, R.O.C.
J Nutr. 1998 Jan;128(1):116-22. doi: 10.1093/jn/128.1.116.
To investigate the effect of dietary oxidized frying oil (OFO) on tissue retention of vitamin C, and to explore the effect of vitamin C supplementation on tissue vitamin E concentrations and lipid peroxidation, male weanling guinea pigs were divided into four groups. Guinea pigs were fed 15% OFO diets supplemented with vitamin C at 300, 600 or 1500 mg/kg diet. Control animals were fed a diet containing 15% fresh untreated soybean oil with 300 mg/kg of vitamin C. After 60 d of feeding, body weight gain, food intake, feed efficiency and plasma triglyceride concentration were significantly lower in guinea pigs fed OFO diets than in controls (P < 0.05). However, plasma cholesterol concentration was highest in guinea pigs fed the OFO diet supplemented with 300 mg/kg vitamin C. Increasing vitamin C in OFO diets significantly reduced plasma cholesterol concentration. Plasma and tissue vitamins C and E concentrations were significantly lower in the OFO-fed guinea pigs receiving 300 mg/kg vitamin C than in controls. Greater levels of supplemental vitamin C increased tissue vitamins C and E. Guinea pigs fed OFO diets had significantly higher tissue levels of thiobarbituric acid reactive substances (TBARS) (P < 0.05) than controls. Our results demonstrated that OFO feeding, which impaired alpha-tocopherol retention and increased TBARS, could be alleviated somewhat by vitamin C supplementation.
为研究膳食氧化煎炸油（OFO）对维生素C组织留存的影响，并探讨补充维生素C对组织维生素E浓度及脂质过氧化的影响，将雄性断奶豚鼠分为四组。给豚鼠喂食添加了300、600或1500mg/kg日粮维生素C的15%OFO日粮。对照动物喂食含有15%新鲜未处理大豆油及300mg/kg维生素C的日粮。喂食60天后，喂食OFO日粮的豚鼠体重增加、食物摄入量、饲料效率及血浆甘油三酯浓度显著低于对照组（P<0.05）。然而，喂食添加300mg/kg维生素C的OFO日粮的豚鼠血浆胆固醇浓度最高。在OFO日粮中增加维生素C可显著降低血浆胆固醇浓度。接受300mg/kg维生素C的OFO喂养豚鼠的血浆及组织维生素C和E浓度显著低于对照组。更高水平的补充维生素C可提高组织维生素C和E。喂食OFO日粮的豚鼠组织中硫代巴比妥酸反应性物质（TBARS）水平显著高于对照组（P<0.05）。我们的结果表明，OFO喂养会损害α-生育酚留存并增加TBARS，补充维生素C可在一定程度上缓解这种情况。
