Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Mar Biotechnol (NY). 2010 Jun;12(3):282-91. doi: 10.1007/s10126-009-9216-z. Epub 2009 Jul 10.
The Agamaki clam (Sinonovacula constricta) is an economically important shellfish in Asia. However, genomic research on this species is still in its infancy, and genomic resources are largely unavailable. The objective of this study was to generate expressed sequence tags (ESTs) from a normalized liver complementary DNA library and to identify genes that function in immune defense. A total of 5,296 ESTs were sequenced, from which 540 contigs and 3,473 singletons were identified. BLAST homology analysis indicated that only 20.7% of these ESTs were homologues of known genes while the remaining 79.3% appeared to be novel sequences. Based on sequence similarities, 43 putative immune genes were identified that encode proteases and protease inhibitors, adhesive proteins, stress proteins, lysosomal enzymes, and signal transduction regulators. Our study thus provides both a large collection of novel transcripts and a detailed annotation of immune genes for an important bivalve species.
方格星虫（Sinonovacula constricta）是亚洲地区一种具有重要经济价值的贝类。然而，针对该物种的基因组研究仍处于起步阶段，基因组资源也非常有限。本研究旨在从一个正常化的肝 cDNA 文库中生成表达序列标签（EST），并鉴定参与免疫防御的基因。共测序了 5296 条 EST，从中鉴定出 540 个重叠群和 3473 个单序列。BLAST 同源性分析表明，这些 EST 中只有 20.7%与已知基因具有同源性，而其余 79.3%似乎是新序列。根据序列相似性，鉴定出 43 个可能的免疫基因，它们编码蛋白酶和蛋白酶抑制剂、黏附蛋白、应激蛋白、溶酶体酶和信号转导调节剂。因此，本研究为一种重要的双壳贝类提供了大量新的转录本和详细的免疫基因注释。
