Zhang Hui, Gong Huanran, Zhou Xueping
Key Laboratory of Molecular Biology of Crop Pathogens and Insects of MOA, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China.
Virus Genes. 2009 Oct;39(2):249-55. doi: 10.1007/s11262-009-0384-8.
Several tomato production regions in China were surveyed for tomato yellow leaf curl disease (TYLCD), and 31 tomato leaf samples showing TYLCD-like symptoms were collected. The partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (TYLCV) was detected in Shanghai, Zhejiang, Jiangsu Shandong and Hebei provinces of China. The TYLCV isolates found in China share high sequence identity ([98%) and have more than 97% sequence identity with TYLCVIL[ IL:Reo] (X15656). Phylogenetic relationship analysis reveals that although with little genetic variability, they can form two groups and all the TYLCV isolates in China belong to the group I. An infectious clone of TYLCV-[CN:SH2] (AM282874) was constructed and agro-inoculated into Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Solanum lycopersicum, Petunia hybrida, Cucumis sativus, Gossypium hirsutum, S. melongena, and Capsicum annuum. TYLCV-[CN:SH2] can induce severe leaf curling and stunting symptoms in these plants except C. sativus, G. hirsutum, S. melongena and C. annuum.We verified that TYLCV can trans-replicate tomato yellow leaf curl China virus DNA-b in N. benthamiana and S. lycopersicum and induced more severe symptoms with distortion and yellow vein.
对中国几个番茄产区进行了番茄黄化曲叶病（TYLCD）调查，收集了31份表现出类似TYLCD症状的番茄叶片样本。对这些分离株的部分或全长基因组进行了测序，在中国上海、浙江、江苏、山东和河北省检测到番茄黄化曲叶病毒（TYLCV）。在中国发现的TYLCV分离株具有较高的序列同一性（>98%），与TYLCVIL[IL:Reo]（X15656）的序列同一性超过97%。系统发育关系分析表明，尽管遗传变异性较小，但它们可形成两个组，中国所有的TYLCV分离株均属于I组。构建了TYLCV-[CN:SH2]（AM282874）的感染性克隆，并通过农杆菌介导接种到本氏烟草、烟草品种Samsun、黏毛烟草、番茄、矮牵牛、黄瓜、陆地棉、茄子和辣椒中。除黄瓜、陆地棉、茄子和辣椒外，TYLCV-[CN:SH2]可在这些植物中诱导严重的叶片卷曲和矮化症状。我们证实，TYLCV可在本氏烟草和番茄中反式复制中国番茄黄化曲叶病毒DNA-b，并诱导更严重的症状，包括变形和黄脉。
