Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, The People's Republic of China.
Vet Res Commun. 2009 Dec;33(8):855-63. doi: 10.1007/s11259-009-9234-3.
To perform phylogenetic analysis of Mycoplasma suis isolates derived from China to define the nature of this pathogen, nearly complete of 16S rRNA genes from Chongqing, Sichuan, Henan and Guangdong isolates were amplified by PCR and sequenced. The four sequences from the blood samples in this study, with other 17 Hemoplasmas sequences and related 3 mycoplasma sequences available in the GenBank, were aligned using Clustal X (version 1.83) sequences alignment program. Maximum parsimony, neighbor-joining and minimum evolution (MEGA 4.0) algorithms were used to create phylogenetic trees. Phylogenetic analysis of these sequences showed that all hemoplasma species were located within a single clade and were most closely related to M. pneumoniae group. The hemoplasma species were further subdivided into two distinct groups, one containing M.wenyonii, M.suis and Candidatus M. haemominutum and the other containing M. haemofelis and M. haemocanis. Within the former clade, four M.suis isolates from Mainland China and other M.suis species formed a monophyletic group in the tree. A tendency of clear geographical grouping of the isolate was evident.
为了对源自中国的猪支原体分离株进行系统发育分析，以明确该病原体的性质，我们通过 PCR 扩增并测序了来自重庆、四川、河南和广东分离株的近乎完整的 16S rRNA 基因。本研究中来自血液样本的 4 个序列，与 GenBank 中其他 17 个血原体序列和相关的 3 个支原体序列一起，使用 Clustal X（版本 1.83）序列比对程序进行了比对。最大简约法、邻接法和最小进化法（MEGA 4.0）算法被用于构建系统发育树。这些序列的系统发育分析表明，所有血原体种均位于单个分支内，与肺炎支原体群最为密切相关。血原体种进一步细分为两个不同的组，一组包含 M. wenyonii、M. suis 和 Candidatus M. haemominutum，另一组包含 M. haemofelis 和 M. haemocanis。在前者的分支内，中国大陆的 4 株猪支原体分离株和其他猪支原体种在树中形成一个单系群。分离株明显存在清晰的地理分组趋势。
