Fu Yuan, Shi Tuanyuan, Xu Lihua, Wei Wei, Lu Fuzhuang, Zhang Xuejuan, Yuan Xiufang, Li Junxing, Lv Jinhui, Fang Weihuan
Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Institute of Animal Husbandry and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, China.
J Vet Med Sci. 2017 May 18;79(5):864-870. doi: 10.1292/jvms.16-0545. Epub 2017 Apr 3.
Hemoplasmas belong to Mycoplasmataceae (Mollicutes: Mycoplasmatales) and are able to infect a broad range of mammalian species. We investigated prevalence of hemotropic mycoplasma species in pig farms in the region of Zhejiang by a PCR scheme using universal primers targeting 16S rRNA and RNase P RNA gene (rnpB). Representative positive samples from different farms were selected for sequencing of 16S rRNA and the 219bp rnpB gene fragments for phylogenetic analysis. Sequencing analysis of PCR products from first samples identified a novel hemoplasma species present in several pig farms in the region with highest nucleotide identity of 92% to Candidatus Mycoplasma turicensis. A duplex PCR assay was then designed for differential detection of the novel hemoplasma from Mycoplasma parvum/M. suis in field samples. Of 324 blood samples from clinically healthy pigs, 26.5% was positive for this novel hemoplasma species and 50% positive for M. suis/M. parvum, indicating that the novel hemotropic mycoplasma species were of considerably high prevalence in Zhejiang province, China.
血支原体属于支原体科（柔膜菌纲：支原体目），能够感染多种哺乳动物。我们通过一种PCR方案，使用靶向16S rRNA和核糖核酸酶P RNA基因（rnpB）的通用引物，调查了浙江省养猪场中嗜血性支原体物种的流行情况。从不同猪场选取代表性阳性样本，对16S rRNA和219bp rnpB基因片段进行测序，用于系统发育分析。对首批样本的PCR产物进行测序分析，鉴定出该地区多个猪场中存在一种新型血支原体物种，其与猪鼻支原体（暂定种）的核苷酸同一性最高为92%。然后设计了一种双重PCR检测方法，用于从田间样本中区分新型血支原体与微小支原体/猪支原体。在324份临床健康猪的血液样本中，26.5%对这种新型血支原体物种呈阳性，50%对猪支原体/微小支原体呈阳性，表明这种新型嗜血性支原体物种在中国浙江省的流行率相当高。
