Dunn James C Y, Chu Yinting, Qin Harry H, Zupekan Tatiana
Department of Surgery and Department of Bioengineering, Division of Pediatric Surgery, Biomedical Engineering Interdepartmental Program, University of California, Los Angeles, California 90095-7098, USA.
J Surg Res. 2009 Oct;156(2):317-24. doi: 10.1016/j.jss.2009.04.021. Epub 2009 May 14.
The adrenal cortex may contain progenitor cells useful for tissue regeneration. Currently there are no established methods to isolate these cells.
Murine adrenal cells were sorted into a Nile-red-bright (NR(bright)) and a Nile-red-dim (NR(dim)) population of cells according to their degree of cholesterol content revealed by Nile red fluorescence. The cells were transplanted under the renal capsule to determine their ability for regeneration.
The NR(bright) cells contained an abundance of lipid droplets, whereas the NR(dim) cells contained little. The NR(bright) cells expressed Sf1 and the more differentiated adrenal cortical genes, including Cyp11a1, Cyp11b1, and Cyp11b2, whereas the NR(dim) cells expressed Sf1 but not the more differentiated adrenal cortical genes. After 56 d of implantation in unilateral adrenalectomized mice, the NR(dim) cells expressed Sf1 and the more differentiated adrenal cortical genes, whereas the NR(bright) cells ceased to express Sf1 as well as the more differentiated adrenal cortical genes. NR(dim) cells also proliferated in the presence of basic fibroblast growth factor.
The population of NR(dim) cells contained adrenal cortical progenitor cells that can proliferate and give rise to differentiated daughter cells. These cells may be useful for adrenal cortical regeneration.
肾上腺皮质可能含有对组织再生有用的祖细胞。目前尚无分离这些细胞的确立方法。
根据尼罗红荧光显示的胆固醇含量程度，将小鼠肾上腺细胞分为尼罗红明亮（NR(bright)）和尼罗红暗淡（NR(dim)）细胞群体。将这些细胞移植到肾被膜下以确定其再生能力。
NR(bright)细胞含有大量脂滴，而NR(dim)细胞几乎不含。NR(bright)细胞表达Sf1以及更分化的肾上腺皮质基因，包括Cyp11a1、Cyp11b1和Cyp11b2，而NR(dim)细胞表达Sf1但不表达更分化的肾上腺皮质基因。在单侧肾上腺切除的小鼠中植入56天后，NR(dim)细胞表达Sf1以及更分化的肾上腺皮质基因，而NR(bright)细胞停止表达Sf1以及更分化的肾上腺皮质基因。NR(dim)细胞在碱性成纤维细胞生长因子存在的情况下也会增殖。
NR(dim)细胞群体含有肾上腺皮质祖细胞，这些祖细胞能够增殖并产生分化的子代细胞。这些细胞可能对肾上腺皮质再生有用。
