Tabakoff B, Hoffman P, Moses F
J Pharm Pharmacol. 1977 Aug;29(8):471-6. doi: 10.1111/j.2042-7158.1977.tb11371.x.
Cessation of chronic ethanol administration, and elimination of ethanol from the body, results in a withdrawal syndrome in mice characterized by behavioural symptoms and hypothermia. During withdrawal, the accumulation rate of [14C] 5-hydroxytryptamine (5-HT) from [14C]tryptophan, was significantly lower in the brainstem of the ethanol-withdrawn animals than in controls. A similar pattern was seen in forebrain. When the rate of 5-HT accumulation was determined using pargyline, no differences occurred between control and ethanol-treated animals. The endogenous concentrations of tryptophan in plasma, and tryptophan, 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) in brain were the same in ethanol-treated and control animals. It is suggested that the changes in accumulation of 14C-5-HT and 14C-5-HIAA in ethanol-withdrawn animals reflected alterations in electrical activity of serotoninergic neurons during withdrawal.
停止长期给予乙醇并将乙醇从体内清除后，小鼠会出现戒断综合征，其特征为行为症状和体温过低。在戒断期间，乙醇戒断动物脑干中由[14C]色氨酸生成[14C]5-羟色胺（5-HT）的积累速率显著低于对照组。前脑也观察到类似模式。当使用帕吉林测定5-HT积累速率时，对照动物和乙醇处理动物之间没有差异。乙醇处理动物和对照动物血浆中色氨酸的内源性浓度以及脑中色氨酸、5-HT和5-羟吲哚乙酸（5-HIAA）的浓度相同。提示乙醇戒断动物中14C-5-HT和14C-5-HIAA积累的变化反映了戒断期间5-羟色胺能神经元电活动的改变。
