谷胱甘肽S-转移酶P1基因多态性及其与胃肠化生癌变风险的相关性

[Polymorphism of glutathione S-transferase P1 and correlation there of with carcinogenesis risk of gastric intestinal metaplasia].

作者信息

Wang Xu-guang, Zhang Zhong, Zhang Ye, Yuan Yuan

机构信息

Institute of General Surgery & Cancer Research, Department of Cancer Control, First Hospital of China Medical University, Shenyang 110001, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2009 Mar 10;89(9):582-6.

PMID:19595155

Abstract

OBJECTIVE

To investigate the distribution of polymorphism of glutathione S-transferase P1 (GSTP1) in different kinds gastric intestinal metaplasia (IM), and the carcinogenesis risk of different types of IM.

METHODS

Peripheral blood samples were collected from 87 gastric cancer patients, 87 intestinal metaplasia patients, and 87 healthy persons as controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze the distribution of different alleles of GSTP1. Biopsy specimens of gastric mucous membrane were collected by gastroscopy. Histochemical staining for mucin was used to identify the kinds of IM.

RESULTS

The G allele rates of the IM and gastric cancer groups were both significantly higher than that of the control group (chi2=6.921, P=0.009; chi2=28.787, P=0.000). The risk of IM of those with G allele was 1.944 times higher then that of the normal controls (95% CI: 1.177-3.209), and the risk of gastric cancer of those with G allele was 3.605 times higher (95% CI: 2.217-5.863). The G allele rate of the gastric cancer group was significantly higher than that of he IM group (chi2=7.687, P=0.006). The G allele rates of the type II and III IM were significantly higher than that of the normal controls (chi2=9.981, P=0.001; chi2=8.845, P=0.002). The risk of type II IM of the subjects with G allele was 2.747 times higher than that of the normal controls (95% CI: 1.475-5.114), and the risk of type III IM of the subjects with G allele was 3.451 times higher (95% CI: 1.556-7.657). The risks of type IIIM, type III IM, and gastric cancer of the subjects with allele G of GSTP1 gene were 2.905, 3.650, and 3.813 times higher than those of the normal controls respectively (95% CI: 1.341-6.293, 1.455-9.153 and 1.953-7.444 respectively).

CONCLUSION

The individuals with G allele show an increased risk of developing IM and gastric cancer, especially type II and III IM. IM patients with G allele have the genetic characteristics similar to those of gastric cancer, so they should be regarded as high-risk individuals of gastric cancer and followed up regularly.

摘要

目的

探讨谷胱甘肽S-转移酶P1（GSTP1）基因多态性在不同类型胃黏膜肠化生（IM）中的分布情况，以及不同类型IM的致癌风险。

方法

收集87例胃癌患者、87例肠化生患者及87例健康人外周血样本作为对照。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析GSTP1不同等位基因的分布。通过胃镜采集胃黏膜活检标本，采用黏液组织化学染色鉴定IM类型。

结果

IM组和胃癌组的G等位基因频率均显著高于对照组（χ2=6.921，P=0.009；χ2=28.787，P=0.000）。携带G等位基因者发生IM的风险是正常对照组的1.944倍（95%可信区间：1.177-3.209），发生胃癌的风险是正常对照组的3.605倍（95%可信区间：2.217-5.863）。胃癌组的G等位基因频率显著高于IM组（χ2=7.687，P=0.006）。Ⅱ型和Ⅲ型IM的G等位基因频率显著高于正常对照组（χ2=9.981，P=0.001；χ2=8.845，P=0.002）。携带G等位基因者发生Ⅱ型IM的风险是正常对照组的2.747倍（95%可信区间：1.475-5.114），发生Ⅲ型IM的风险是正常对照组的3.451倍（95%可信区间：1.556-7.657）。携带GSTP1基因G等位基因者发生Ⅲ型胃黏膜异型增生、Ⅲ型IM和胃癌的风险分别是正常对照组的2.905倍、3.650倍和3.813倍（95%可信区间分别为：1.341-6.293、1.455-9.153和1.953-7.444）。