Borres M P, Ahlstedt S, Björkstén B
Department of Pediatrics, University of Linköping, Uppsala, Sweden.
Int Arch Allergy Appl Immunol. 1991;95(4):289-93. doi: 10.1159/000235463.
The appearance of basophilic cells by maturation from progenitors was studied in cultures of cord blood mononuclear cells (CB-MNC) from 83 newborns. In unstimulated cultures, the numbers of basophilic cells increased 5-fold after 14 days of cultivation. This increase and the cell numbers were similar in cultures from atopic individuals and controls. Other cultures were stimulated with conditioned medium (CM) prepared from 3-day cultures of blood cells from atopic individuals activated with allergen or with CM from the Mo leukemic cell line. The addition of such CM resulted in an increase in the number of basophilic cells, as compared to the cultures which were unstimulated (p less than 0.01). No correlation was found between the maturation of basophils and heredity for atopy, development of atopic disease, appearance of metachromatic cells in the nasal mucosa and perinatal factors, such as maternal infections, perinatal stress, birth weight, month of birth and IgE levels. The study confirmed that basophilic cells can be driven to mature in cultures of CB-MNC, although the maturation did not seem to depend on atopic predisposition as indicated by family history of allergy and CB IgE levels nor on symptoms and signs of atopic disease during the first 18 months of life. Therefore, the predictive value of elevated basophilic cell numbers in CB or CB-MNC cultures with regard to allergic disease seemed to be limited.
在来自83名新生儿的脐血单个核细胞（CB-MNC）培养物中，研究了祖细胞成熟产生嗜碱性细胞的情况。在未刺激的培养物中，培养14天后嗜碱性细胞数量增加了5倍。在特应性个体和对照组的培养物中，这种增加以及细胞数量相似。其他培养物用由变应原激活的特应性个体血细胞的3天培养物制备的条件培养基（CM）或Mo白血病细胞系的CM进行刺激。与未刺激的培养物相比，添加这种CM导致嗜碱性细胞数量增加（p小于0.01）。未发现嗜碱性粒细胞的成熟与特应性的遗传、特应性疾病的发展、鼻黏膜中异染细胞的出现以及围产期因素（如母亲感染、围产期应激、出生体重、出生月份和IgE水平）之间存在相关性。该研究证实，在CB-MNC培养物中嗜碱性细胞可以被驱动成熟，尽管成熟似乎不依赖于过敏家族史和CB IgE水平所表明的特应性易感性，也不依赖于生命最初18个月内特应性疾病的症状和体征。因此，CB或CB-MNC培养物中嗜碱性细胞数量升高对于过敏性疾病的预测价值似乎有限。
