Ahlstedt S, Hammarström I, Into-Malmberg M B, Björkstén B, Denburg J
Int Arch Allergy Appl Immunol. 1986;79(1):33-7. doi: 10.1159/000233938.
The appearance of large human basophilic cells containing histamine was followed to reflect cell maturation in cultures of peripheral blood leukocytes. Aliquots were taken on day 0 and after 7, 9-10 and 14 days in culture, and the basophilic cell numbers were determined in 200 microliter cytospin specimens stained with Wright's stain. The number of small basophils initially present declined during the culture period. The number of spontaneously appearing large basophilic cells varied among the cultures from different individuals and was slightly, though not significantly, higher in atopic individuals than in normals. In cultures of cells from atopic individuals, the number of large basophilic cells was reproducibly increased by the addition of appropriate allergen to the cultures. In cultures of cells from normal individuals, the number was increased two- to six-fold by adding conditioned medium (CM), either from 3-day cultures of cells from atopic individuals stimulated with allergen or with CM from the Mo T cell leukemic line. The addition of mitomycin C decreased the number of large basophilic cells and cycloheximide abrogated their appearance.
通过观察含有组胺的大型人类嗜碱性细胞的出现来反映外周血白细胞培养物中的细胞成熟情况。在培养的第0天以及培养7天、9 - 10天和14天后取等分试样，并用瑞氏染色法在200微升细胞涂片标本中测定嗜碱性细胞数量。最初存在的小嗜碱性粒细胞数量在培养期间减少。自发出现的大型嗜碱性细胞数量在来自不同个体的培养物中有所不同，特应性个体中的数量略高于正常人，但差异不显著。在来自特应性个体的细胞培养物中，向培养物中添加适当的变应原可使大型嗜碱性细胞数量重复性增加。在来自正常个体的细胞培养物中，添加条件培养基（CM）可使数量增加2至6倍，该条件培养基要么来自用变应原刺激的特应性个体细胞的3天培养物，要么来自Mo T细胞白血病系的条件培养基。添加丝裂霉素C可减少大型嗜碱性细胞的数量，而环己酰亚胺可消除它们的出现。
