Sugito S, Yamato K, Sameshima Y, Yokota J, Yano S, Miyoshi I
Department of Medicine, Kochi Medical School, Japan.
Int J Cancer. 1991 Dec 2;49(6):880-5. doi: 10.1002/ijc.2910490614.
Experiments were undertaken to investigate a genetic event involved in leukemogenesis in adult T-cell leukemia (ATL). For this purpose, the p53 gene was chosen for study, since alteration of the gene has been found in a wide variety of human cancers. Structures and expression of the p53 gene in ATL cells were investigated by Southern and Northern blot analyses and a polymerase-chain-reaction single-strand conformation-polymorphism (PCR-SSCP) analysis. Either subtle alterations of the p53 gene or the absence of detectable level of p53 mRNA were found in 2 of 3 acute ATL cell lines and 2 of 12 acute ATL fresh samples. In contrast, no mutation was detected in 4 cases with less aggressive types of ATL (3 chronic and 1 smoldering ATL cases). Mutations found in acute ATL cells occurred in regions highly conserved in evolution and all the cells carrying p53 mutation showed loss of the other p53 allele. These results suggests that alteration of the p53 gene may contribute to progression of the disease in some cases of ATL.
开展了实验以研究成人T细胞白血病（ATL）白血病发生过程中涉及的一个基因事件。为此，选择p53基因进行研究，因为已在多种人类癌症中发现该基因的改变。通过Southern印迹分析、Northern印迹分析以及聚合酶链反应单链构象多态性（PCR-SSCP）分析，研究了ATL细胞中p53基因的结构和表达。在3个急性ATL细胞系中的2个以及12个急性ATL新鲜样本中的2个中，发现了p53基因的细微改变或未检测到可检测水平的p53 mRNA。相比之下，在4例侵袭性较小的ATL类型（3例慢性和1例冒烟型ATL病例）中未检测到突变。在急性ATL细胞中发现的突变发生在进化上高度保守的区域，并且所有携带p53突变的细胞均显示另一个p53等位基因缺失。这些结果表明，在某些ATL病例中，p53基因的改变可能促进疾病进展。
