Hod M, Dorsman M, Friedman S, Ovadia J, Reisner S, Lapidot A
Department of Obstetrics and Gynecology, Beilinson Medical Center, Petah Tikva, Israel.
Isr J Med Sci. 1991 Aug-Sep;27(8-9):462-8.
This study presents kinetic parameters of glycine metabolism during pregnancy and the influence of fuel availability in the maternal compartment. The effect of gestational diabetes mellitus (diet treated) and pre-gestational diabetes mellitus (diet and insulin treated), accompanied by increased fetal growth on [15N]glycine kinetic parameters, compared to normal pregnancies with normal fetal growth, has been determined. This process was measured by administration of a single-dose of [15N]glycine to post-absorptive normal and diabetic pregnant women during the third trimester of pregnancy. Gas chromatography-mass spectrometry was used to determine the 15N enrichment of plasma glycine, and to calculate the pool sizes, turnover rate constants, fluxes and metabolic clearance rates. Glycine pool sizes in pre-gestational diabetes were significantly larger than those in normal pregnancy and gestational diabetes. Glycine turnover rate constants and metabolic clearance rates were not significantly different between the normal pregnant women and the two diabetic groups of pregnant women. Glycine fluxes were significantly higher in the pre-gestational diabetic pregnant women than in gestational diabetes and normal pregnancy. The pre-gestational diabetic pregnant women delivered fetuses with higher birth weights than the other two groups.
本研究呈现了孕期甘氨酸代谢的动力学参数以及母体代谢池燃料供应情况的影响。与胎儿生长正常的正常妊娠相比，已确定妊娠糖尿病（饮食治疗）和孕前糖尿病（饮食及胰岛素治疗）伴胎儿生长加速对[15N]甘氨酸动力学参数的影响。此过程通过在妊娠晚期对吸收后状态的正常和糖尿病孕妇单次给予[15N]甘氨酸来测量。采用气相色谱 - 质谱法测定血浆甘氨酸的15N富集度，并计算代谢池大小、周转率常数、通量和代谢清除率。孕前糖尿病患者的甘氨酸代谢池大小显著大于正常妊娠和妊娠糖尿病患者。正常孕妇与两组糖尿病孕妇之间的甘氨酸周转率常数和代谢清除率无显著差异。孕前糖尿病孕妇的甘氨酸通量显著高于妊娠糖尿病孕妇和正常孕妇。孕前糖尿病孕妇分娩的胎儿出生体重高于其他两组。
