Toescu V, Nuttall S L, Martin U, Nightingale P, Kendall M J, Brydon P, Dunne F
Division of Medical Sciences, The Medical School, University of Birmingham, Edgbaston, Birmingham B29 6JD, UK.
Clin Sci (Lond). 2004 Jan;106(1):93-8. doi: 10.1042/CS20030175.
The purpose of the present study was to determine changes in plasma lipids and markers of oxidative stress longitudinally in pregnancy complicated by diabetes compared with non-diabetic pregnancy. This was carried out by following a group of normal pregnant women (n=17) and groups of pregnant women with Type I diabetes (n=19), Type II diabetes (n=12) and gestational diabetes mellitus (n=12) throughout pregnancy, with sampling carried out at the end of each trimester. Serum total cholesterol and triacylglycerols (triglycerides) were determined using standard colorimetric techniques and low-density lipoprotein (LDL) subfraction profile by disc PAGE. Total antioxidant capacity (TAC) was determined by enhanced chemiluminescence and lipid hydroperoxides by the ferrous oxidation of Xylenol Orange method. Total cholesterol and triacylglycerols increased significantly throughout pregnancy in all groups, but there were no significant differences between normal and diabetic women with respect to either. The LDL score was significantly higher (P<0.001) in diabetic women compared with normal women at each point throughout pregnancy, although there were no significant differences between the diabetic groups. There was evidence of greater oxidative stress in diabetic compared with normal women throughout. Corrected TAC was significantly lower (P<0.001) in all diabetic women throughout pregnancy. In addition, lipid hydroperoxides were higher in all diabetic compared with normal women, particularly so in those with Type II diabetes (P<0.05). These changes may have important implications for diabetic women during pregnancy, as an elevated risk of pre-eclampsia is thought to reflect an oxidative stress-related mechanism. In addition, these changes may have important implications for the development of atherosclerosis and the long-term cardiovascular health of women with diabetes.
本研究的目的是纵向确定与非糖尿病妊娠相比，糖尿病合并妊娠时血浆脂质和氧化应激标志物的变化。通过对一组正常孕妇（n = 17）以及患有I型糖尿病（n = 19）、II型糖尿病（n = 12）和妊娠期糖尿病（n = 12）的孕妇群体在整个孕期进行跟踪，并在每个孕期末进行采样来实现这一目的。使用标准比色技术测定血清总胆固醇和三酰甘油（甘油三酯），并通过圆盘聚丙烯酰胺凝胶电泳测定低密度脂蛋白（LDL）亚组分谱。通过增强化学发光法测定总抗氧化能力（TAC），通过二甲苯酚橙亚铁氧化法测定脂质氢过氧化物。所有组的总胆固醇和三酰甘油在整个孕期均显著增加，但正常孕妇和糖尿病孕妇在这两方面均无显著差异。在整个孕期的每个时间点，糖尿病孕妇的LDL评分均显著高于正常孕妇（P < 0.001），尽管糖尿病组之间无显著差异。有证据表明，与正常孕妇相比，糖尿病孕妇在整个孕期的氧化应激更大。所有糖尿病孕妇在整个孕期校正后的TAC均显著较低（P < 0.001）。此外，与正常孕妇相比，所有糖尿病孕妇的脂质氢过氧化物均较高，尤其是II型糖尿病患者（P < 0.05）。这些变化可能对糖尿病孕妇在孕期具有重要意义，因为子痫前期风险升高被认为反映了一种与氧化应激相关的机制。此外，这些变化可能对动脉粥样硬化的发展以及糖尿病女性的长期心血管健康具有重要意义。
