Ghasemi N, Babaei H, Azizallahi S, Kheradmand A
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Andrologia. 2009 Aug;41(4):222-8. doi: 10.1111/j.1439-0272.2009.00920.x.
This study was set to investigate whether the adverse effects of heat on spermatozoa and subsequent foetuses could be prevented by long-term zinc administration. The scrotums of animals were immersed in water at either 43 degrees C (heat group) or 23 degrees C (control group). Half of the heat and control mice were given 10 mg kg(-1) zinc every other day for 60 days and the others received sterile saline instead of zinc. Heat stress significantly reduced sperm motility, concentration, hypoosmotic swelling-water test (HOS-WT) positive and chromomycin A(3) (CMA(3)) negative spermatozoa at the first 15 days, and the greatest decrease occurred at 30 days (P < 0.05). Sperm motility, concentration and HOS-WT positive spermatozoa were also reduced initially in the zinc administered group, but we did not observe any further decrease in the above mentioned parameters on day 30 (P < 0.05). The weight of foetuses obtained from the females mated with paternal heat treatment males was significantly lower than that of the control group (P < 0.05) and long-term zinc therapy caused a partial recovery (P > 0.05). This study demonstrates that the adverse effects of hyperthermia on semen parameters may be prevented by zinc therapy. Likewise, long-term administration of zinc could improve quality of litter obtained from the females mated with scrotal heat treatment males.
本研究旨在调查长期补锌是否可以预防高温对精子及后续胎儿的不良影响。将动物的阴囊浸入43摄氏度的水中（高温组）或23摄氏度的水中（对照组）。高温组和对照组的小鼠各有一半每隔一天给予10 mg kg(-1)的锌，持续60天，其余小鼠则给予无菌生理盐水而非锌。在最初的15天，热应激显著降低了精子活力、浓度、低渗肿胀试验（HOS-WT）阳性和嗜铬霉素A(3)（CMA(3)）阴性精子的数量，在第30天下降最为明显（P < 0.05）。补锌组的精子活力、浓度和HOS-WT阳性精子数量最初也有所下降，但在第30天我们未观察到上述参数有进一步下降（P < 差异无统计学意义）。与接受父本热应激处理的雄性小鼠交配的雌性小鼠所产胎儿的体重显著低于对照组（P < 0.05），而长期补锌治疗可使体重部分恢复（P > 0.05）。本研究表明，锌疗法可能预防高温对精液参数的不良影响。同样，长期补锌可以提高与接受阴囊热应激处理的雄性小鼠交配的雌性小鼠所产仔畜的质量。
