Departments of Pathology, Hospital Clínic, Institut d'Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain.
HIV Med. 2009 Nov;10(10):634-9. doi: 10.1111/j.1468-1293.2009.00737.x. Epub 2009 Jul 6.
Neoplasms associated with human papillomavirus (HPV) infection occur at increased frequency in patients with HIV infection/AIDS. Although laryngeal squamous cell carcinomas (LSCCs) in HIV-positive patients are uncommon, a higher incidence of this malignancy in HIV-positive patients than in the general population has been reported. As a proportion of LSCCs are associated with HPV in the general population, the clinicopathological features of a series of LSCCs developing in HIV-positive patients were evaluated to investigate the possible relationship with HPV infection, and infection with other oncogenic viruses.
All HIV-positive patients with LSCC diagnosed at a single institution from 1998 to 2007 were retrospectively evaluated. The clinicopathological features were analysed and tissues were tested by polymerase chain reaction (PCR), using the short PCR fragment 10 (SPF10) primer, a highly sensitive method for HPV DNA detection. Immunohistochemical studies for HIV p24, p16(INK4a) and p53 were performed. Epstein-Barr virus (EBV) and human herpes virus 8 (HHV-8) were also investigated.
Six out of 4987 HIV-infected patients seen in this period in the Infectious Diseases Department developed LSCC (median age 41.5 years; male to female ratio 1:1). All patients were heavy smokers and the tumours presented at an advanced clinical stage. HPV was not detected in any tumour, not even in two patients with coexisting HPV-associated gynaecological neoplasm. Staining for HIV p24 and p16(INK4a) was negative, whereas p53 was positive in half the cases. EBV and HHV-8 were also negative.
LSCC developing in HIV-positive patients is an infrequent neoplasm, not usually associated with HPV infection. It develops in young, heavy smokers and presents at an advanced clinical stage.
人乳头瘤病毒（HPV）感染相关的肿瘤在 HIV 感染/艾滋病患者中发病率增高。虽然 HIV 阳性患者的喉鳞状细胞癌（LSCC）并不常见，但 HIV 阳性患者的这种恶性肿瘤发病率高于普通人群。由于普通人群中存在与 HPV 相关的 LSCC，因此评估了一组在 HIV 阳性患者中发生的 LSCC 的临床病理特征，以研究其与 HPV 感染和其他致癌病毒感染的可能关系。
回顾性分析了 1998 年至 2007 年期间在一家机构诊断出的所有 HIV 阳性 LSCC 患者。分析了临床病理特征，并使用聚合酶链反应（PCR）、短 PCR 片段 10（SPF10）引物检测组织，该方法对 HPV DNA 的检测高度敏感。进行了 HIV p24、p16（INK4a）和 p53 的免疫组织化学研究。还对 EBV 和 HHV-8 进行了检测。
在传染病科就诊的 4987 例 HIV 感染患者中，有 6 例（中位年龄 41.5 岁；男女比例 1:1）发生 LSCC。所有患者均为重度吸烟者，肿瘤处于晚期临床阶段。在任何肿瘤中均未检测到 HPV，甚至在 2 例同时存在 HPV 相关妇科肿瘤的患者中也未检测到 HPV。HIV p24 和 p16（INK4a）的染色均为阴性，而 p53 在一半病例中呈阳性。EBV 和 HHV-8 也为阴性。
在 HIV 阳性患者中发生的 LSCC 是一种罕见的肿瘤，通常不与 HPV 感染相关。它发生在年轻、重度吸烟的患者中，且处于晚期临床阶段。
