Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil.
Malar J. 2009 Jul 14;8:156. doi: 10.1186/1475-2875-8-156.
The goal of the present study was the characterization of Plasmodium falciparum genes associated to malaria drug resistance (pfcrt, pfdhfr and pfdhps), in samples from two Brazilian localities.
Parasites from 65 P. falciparum samples were genotyped using nested-PCR and direct DNA sequencing.
Six resistant sulphadoxine-pyrimethamine (SP) pfdhfr genotypes and one haplotype associated to SP sensitivity were detected. For pfcrt gene, SVMNT chloroquine (CQ)-resistant genotype was detected as well as the CVMNK CQ-sensitive haplotype in the same sample from Paragominas, that showed a SP-sensitive genotype.
This study is the first to document the sensitivity of P. falciparum parasites to CQ and SP in Brazilian field samples. The importance of these findings is discussed.
本研究的目的是对来自巴西两个地区的疟原虫样本中的疟疾药物耐药相关基因（pfcrt、pfdhfr 和 pfdhps）进行特征分析。
使用巢式 PCR 和直接 DNA 测序对 65 个恶性疟原虫样本中的寄生虫进行基因分型。
检测到六种对磺胺多辛-乙胺嘧啶（SP）耐药的 pfdhfr 基因型和一种与 SP 敏感性相关的单倍型。对于 pfcrt 基因，在来自帕拉戈米纳斯的同一样本中检测到 SVMNT 氯喹（CQ）耐药基因型以及 CVMNK CQ 敏感单倍型，该样本显示出 SP 敏感基因型。
本研究首次记录了巴西实地样本中恶性疟原虫对 CQ 和 SP 的敏感性。讨论了这些发现的重要性。
