通过将已建立的T细胞系和克隆与W/Fu（C58NT）D融合产生的CD4大鼠×大鼠以及小鼠×大鼠T细胞杂交瘤。
CD4 rat x rat and mouse x rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)D.
作者信息
Boots A M, van Lierop M J, Wauben M H, van Kooten P J, Hensen E J, van Eden W
机构信息
Institute of Molecular Biology and Medical Biotechnology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
出版信息
J Immunol Methods. 1991 Nov 5;144(1):1-10. doi: 10.1016/0022-1759(91)90223-3.
Abstract
Previously, fusion of established T cell lines or clones has been claimed to be difficult. We now report our experiences in the fusion of both long term cultures of rat T cell clones and mouse T cell lines to rat W/Fu (C58NT)D. Upon fusion of rat T cell clones the hybrids obtained expressed antigen specificities identical to those of the parent clones. In addition, C58 was used for interspecies hybridisation of murine T cell lines. The specificity of intra- and inter-species hybrids was maintained by subcloning. We conclude that the C58 cell line can be used to generate continuously growing monoclonal T-cell reagents of sufficient stability using both intra- and inter-species hybridisation.
摘要
此前，已有报道称已建立的T细胞系或克隆的融合存在困难。我们现在报告我们在将大鼠T细胞克隆和小鼠T细胞系的长期培养物与大鼠W/Fu（C58NT）D进行融合方面的经验。大鼠T细胞克隆融合后获得的杂种细胞表达的抗原特异性与亲本克隆相同。此外，C58用于小鼠T细胞系的种间杂交。通过亚克隆维持种内和种间杂种的特异性。我们得出结论，C58细胞系可用于通过种内和种间杂交产生具有足够稳定性的持续生长的单克隆T细胞试剂。
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