Kabiri Zahra, Salehi Mansoor, Mokarian Fariborz, Mohajeri Mohammad Reza, Mahmoodi Farzaneh, Keyhanian Kianoosh, Doostan Iman, Ataollahi Mohammad Reza, Modarressi Mohammad Hossein
Department of Genetics and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
Cell Immunol. 2009;259(2):111-6. doi: 10.1016/j.cellimm.2009.06.004. Epub 2009 Jun 6.
The participation of Abl-Related Gene (ARG) is demonstrated in pathogenesis of different human malignancies. However there is no conclusive evidence on ARG expression level in mature B cell lymphomas. In this study we evaluated ARG protein expression in Follicular Lymphoma (FL), Burkitt's Lymphoma (BL) and Diffused Large B Cell Lymphoma (DLBCL) in comparison with non-neoplastic lymph nodes. Semi-quantitative fluorescent ImmunoHistoChemistry was applied on 14, 7 and 4 patients with DLBCL, FL and BL respectively, adding to 4 normal and 4 reactive lymph nodes. The mean ratio of ARG/GAPDH expression was significantly different (p<0.00) between lymphomas and control samples, with DLBCL having the highest ARG expression amongst all. Over expression of ARG was seen in FL and BL, with FL expressing statistically more ARG than BL. Moreover, the ARG/GAPDH expression ratio increased from DLBCL stage I towards stage VI, all showing significantly more ARG expression than FL and BL (in all cases p<0.00).
Abl相关基因（ARG）参与了多种人类恶性肿瘤的发病机制。然而，关于成熟B细胞淋巴瘤中ARG表达水平尚无确凿证据。在本研究中，我们评估了滤泡性淋巴瘤（FL）、伯基特淋巴瘤（BL）和弥漫性大B细胞淋巴瘤（DLBCL）中ARG蛋白的表达，并与非肿瘤性淋巴结进行比较。分别对14例DLBCL、7例FL和4例BL患者以及4例正常淋巴结和4例反应性淋巴结进行了半定量荧光免疫组织化学检测。淋巴瘤与对照样本之间ARG/GAPDH表达的平均比值存在显著差异（p<0.00），其中DLBCL的ARG表达在所有样本中最高。在FL和BL中观察到ARG过表达，FL的ARG表达在统计学上高于BL。此外，ARG/GAPDH表达比值从DLBCL的I期到VI期逐渐增加，所有阶段的ARG表达均显著高于FL和BL（在所有情况下p<0.00）。
