Hökfelt T, Halasz N, Ljungdahl A, Johansson O, Goldstein M, Park D
Department of Histology, Karolinska Institute, 104 01 Stockholm, Sweden; Department of Psychiatry, Neurochemistry Laboratories, New York Medical Center, New York, N.Y. 10016, U.S.A.
Neurosci Lett. 1975 Aug;1(2):85-90. doi: 10.1016/0304-3940(75)90050-6.
With the Vibratome modification of the Falck-Hillarp technique, and with the indirect immunofluorescence technique for visualizing the first three enzymes in catecholamine synthesis, evidence has been obtained that in the rat olfactory bulb several periglomerular cells are dopaminergic. Both tyrosine hydroxylase and dopadecarboxylase, converting tyrosine to DOPA and DOPA to dopamine (DA), respectively, but not dopamine-beta-hydroxylase, converting DA to noradrenaline, are present in periglomerular cell bodies, as well as in their intraglomerular dendrites. These findings suggest that DA may be active at dendrodentritic synapses known to be present between periglomerular and mitral and/or tufted cells.
通过对福尔克-希拉尔普技术进行振动切片机改良，并采用间接免疫荧光技术来观察儿茶酚胺合成过程中的前三种酶，已获得证据表明，大鼠嗅球中的几个球周细胞是多巴胺能的。将酪氨酸分别转化为多巴以及将多巴转化为多巴胺（DA）的酪氨酸羟化酶和多巴脱羧酶存在于球周细胞体及其球内树突中，但将多巴胺转化为去甲肾上腺素的多巴胺β-羟化酶则不存在。这些发现表明，多巴胺可能在已知存在于球周细胞与僧帽细胞和/或簇状细胞之间的树突-树突突触中发挥作用。
