Davis B J
Department of Cell Biology, University of Alabama, Birmingham 35294.
J Comp Neurol. 1991 Dec 15;314(3):493-511. doi: 10.1002/cne.903140307.
A comparative analysis of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase activity in the olfactory bulb was conducted in the hamster and rat. The distribution and morphological features of NADPH-stained neurons were compared to those of glutamic acid decarboxylase-like (GAD-LI) and tyrosine hydroxylase-like (TH-LI) immunoreactive somata in order to relate NADPH-staining to neuronal classes with specific biochemical properties. Intense NADPH-staining was located in primary nerve fibers of the accessory and main olfactory systems, producing dense staining of individual glomeruli. The entire vomeronasal nerve and all glomeruli were stained in the accessory olfactory bulb, but olfactory nerve and glomerular staining were restricted to the dorsal half of the main olfactory bulb. The glomerular layer of the main olfactory bulb of both animals contained numerous small NADPH-stained neurons. The range of somal areas of these neurons was relatively narrow and averaged about 60 microns2 (ca. 8 x 11 microns). Most neurons possessed ovoid somata and monoglomerular intraglomerular dendrites. Previous Golgi studies indicate that such features characterize periglomerular cells. The somal areas of GAD-LI somata in the glomerular layer overlapped that of the NADPH-stained neurons, providing additional evidence that these neurons are probably periglomerular cells. The range of somal areas of TH-LI somata in the glomerular layer was broader and included both small and large neurons that usually possessed intraglomerular dendritic tufts. The smaller TH-LI somata corresponded in size to both the NADPH-stained and GAD-LI somata, suggesting an interrelationship among periglomerular cells, GAD-LI, TH-LI, and NADPH-diaphorase activity. The larger TH-LI somata were probably external tufted cells. In the external plexiform layer of the hamster, oriented NADPH-stained neurons were observed that possessed an intraglomerular dendrite. These neurons appeared to be middle tufted cells. Lightly stained and smaller neurons were occasionally seen in the mitral body and internal plexiform layers, corresponding in somal area and morphological features to those of type III granule cells. No internal tufted or mitral cells were stained. The largest NADPH-stained neurons were located in the inner half of the granule cell layer and were classified as Golgi cells. Their somata averaged 125 microns2 (ca. 10 x 17 microns). Many NADPH-stained neurons were observed in all subdivisions of the anterior olfactory nucleus, the anterior hippocampal rudiment, anterior and posterior levels of the piriform cortex, and the vertical and horizontal limbs of the diagonal band of Broca, all of which are known to provide centrifugal inputs to the olfactory bulb.(ABSTRACT TRUNCATED AT 400 WORDS)
对仓鼠和大鼠嗅球中的烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-黄递酶活性进行了比较分析。将NADPH染色神经元的分布和形态特征与谷氨酸脱羧酶样（GAD-LI）和酪氨酸羟化酶样（TH-LI）免疫反应性躯体的分布和形态特征进行比较，以便将NADPH染色与具有特定生化特性的神经元类别联系起来。强烈的NADPH染色位于副嗅系统和主嗅系统的初级神经纤维中，使单个肾小球产生密集染色。副嗅球中的整个犁鼻神经和所有肾小球均被染色，但嗅神经和肾小球染色仅限于主嗅球的背侧半部。两种动物主嗅球的肾小球层都含有许多小的NADPH染色神经元。这些神经元的躯体面积范围相对较窄，平均约为60平方微米（约8×11微米）。大多数神经元具有卵圆形的躯体和单肾小球内的树突。先前的高尔基研究表明，这些特征是球周细胞的特征。肾小球层中GAD-LI躯体的面积与NADPH染色神经元的面积重叠，这进一步证明这些神经元可能是球周细胞。肾小球层中TH-LI躯体的面积范围更广，包括通常具有肾小球内树突簇的小神经元和大神经元。较小的TH-LI躯体在大小上与NADPH染色和GAD-LI躯体相对应，表明球周细胞、GAD-LI、TH-LI和NADPH-黄递酶活性之间存在相互关系。较大的TH-LI躯体可能是外侧丛状细胞。在仓鼠的外侧丛状层中，观察到具有肾小球内树突的定向NADPH染色神经元。这些神经元似乎是中间丛状细胞。在二尖瓣体和内侧丛状层中偶尔可见轻度染色且较小的神经元，其躯体面积和形态特征与III型颗粒细胞的相似。没有内侧丛状细胞或二尖瓣细胞被染色。最大的NADPH染色神经元位于颗粒细胞层的内侧半部，被归类为高尔基细胞。它们的躯体平均为125平方微米（约10×17微米）。在前嗅核的所有亚区、前海马原基、梨状皮质的前、后水平以及布洛卡斜带的垂直和水平支中均观察到许多NADPH染色神经元，所有这些区域已知都向嗅球提供离心输入。（摘要截于400字）
