Ministry of Education and Dept. of Pharmaceutical Engineering, Tianjin University, P. R. China.
Biotechnol Prog. 2009 Sep-Oct;25(5):1244-53. doi: 10.1002/btpr.209.
To obtain a better understanding of responsive mechanism of plant cells in response to hydrodynamic mechanical stress, a metabolic profiling approach was used to profile metabolite changes of Taxus cuspidata cells under laminar shear stress. A total of 65 intracellular metabolites were identified and quantified, using gas chromatography coupled to time-of-flight mass spectrometry. Potential biomarkers were found by the principal component analysis as well as partial least squares combined with variable influence in the projection. Trehalose, sorbitol, ascorbate, sucrose, and gluconic acid were mainly responsible for the discrimination between shear stress induced cells and control cells. Further analysis by mapping measured metabolite concentrations onto the metabolic network revealed that shear stress imposed restrictions on primary metabolic pathways by inhibiting tricarboxylic acid cycle, glycolysis, and N metabolism. To adapt to the shear condition, cells responded by starting defensive programs. These defensive programs included coinduction of glycolysis and sucrose metabolism, accumulation of compatible solutes, and antioxidative strategy. A strategy of defense mechanisms at the level of metabolites for T. cuspidata cells when challenged with the shear stress was proposed.
为了更好地了解植物细胞对流体动力机械应激的响应机制，采用代谢组学方法研究了紫杉醇细胞在层流剪切应力下的代谢产物变化。使用气相色谱-飞行时间质谱联用技术鉴定并定量了 65 种细胞内代谢物。通过主成分分析和偏最小二乘结合投影变量影响分析找到了潜在的生物标志物。海藻糖、山梨醇、抗坏血酸、蔗糖和葡萄糖酸主要负责区分剪切应力诱导的细胞和对照细胞。通过将测量的代谢物浓度映射到代谢网络上进行进一步分析表明，剪切应力通过抑制三羧酸循环、糖酵解和 N 代谢来限制初级代谢途径。为了适应剪切条件，细胞通过启动防御程序来做出响应。这些防御程序包括糖酵解和蔗糖代谢的协同诱导、相容性溶质的积累和抗氧化策略。提出了紫杉醇细胞在受到剪切应力时在代谢物水平上的防御机制策略。
