Lin Shuhua, Wang Yuanxiu, Lu Qunlin, Zhang Bin, Wu Xiaoyu
College of Bioscience and Bioengineering, Jiangxi Agriculture University, Nanchang, 330045, China.
Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang, 330045, China.
Bioresour Bioprocess. 2021 Dec 2;8(1):120. doi: 10.1186/s40643-021-00472-5.
Penicillium digitatum is the primary spoilage fungus that causes green mold during postharvest in citrus. To reduce economic losses, developing more efficient and less toxic natural antimicrobial agents is urgently required. We previously found that the X33 antimicrobial oligopeptide (X33 AMOP), produced by Streptomyces lavendulae X33, exhibited a sterilization effect on P. digitatum. In this study, the effects, and physiological mechanisms of X33 AMOP as an inhibitor of P. digitatum were investigated. The transcriptional and metabolome profiling of P. digitatum exposed to X33 AMOP revealed 3648 genes and 190 metabolites that were prominently changed. The omics analyses suggested that X33 AMOP mainly inhibited P. digitatum growth by affecting cell integrity, genetic information delivery, oxidative stress tolerance, and energy metabolism. These findings provide helpful information regarding the antimicrobial mechanism of X33 AMOP against P. digitatum at the molecular level and indicate that X33 AMOP is a potential candidate to control P. digitatum.
指状青霉是柑橘采后导致绿霉病的主要腐败真菌。为减少经济损失，迫切需要开发更高效、毒性更低的天然抗菌剂。我们之前发现，薰衣草链霉菌X33产生的X33抗菌寡肽（X33 AMOP）对指状青霉具有杀菌作用。在本研究中，我们研究了X33 AMOP作为指状青霉抑制剂的作用及其生理机制。对暴露于X33 AMOP的指状青霉进行转录组和代谢组分析，发现有3648个基因和190种代谢物发生了显著变化。组学分析表明，X33 AMOP主要通过影响细胞完整性、遗传信息传递、氧化应激耐受性和能量代谢来抑制指状青霉的生长。这些发现为X33 AMOP在分子水平上对指状青霉的抗菌机制提供了有用信息，并表明X33 AMOP是控制指状青霉的潜在候选物。
