Zhong Cheng, Yang Song, Yuan Ying-Jin
Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, People's Republic of China.
Biotechnol Appl Biochem. 2009 Jun 22;53(Pt 4):265-75. doi: 10.1042/BA20080203.
Molecular responses of phospholipids of Taxus cuspidata (Japanese yew) cells to hydrodynamic shear stress were investigated by using two bubble columns with different nozzle sizes. T. cuspidata cells cultured in bubble columns responded with an increase in cell membrane permeability when compared with shake-flask culture, corresponding to the alteration of phospholipid constitutions. Phospholipid differences between bubble columns and shake-flask cultures were analysed by using LC/ESI (liquid chromatography/electrospray ionization)-MS coupled with PCA (principal component analysis). It was found that PA (phosphatidic acid) is the main molecular species of potential biomarkers. The amount of PA was found to increase, whereas PC (phosphatidylcholine) and PI (phosphatidylinositol) decreased after 96 h of cultivation in bubble columns, corresponding to the obvious increase in membrane permeability after 96 h of culture in such columns. A decrease in the nozzle size of the bubble column led to a more significant difference in phospholipids. These results suggest that PA accumulation plays an important role in the membrane degradation induced by hydrodynamic shear stress.
利用两个具有不同喷嘴尺寸的鼓泡塔，研究了东北红豆杉细胞磷脂对流体动力剪切应力的分子响应。与摇瓶培养相比，在鼓泡塔中培养的东北红豆杉细胞表现出细胞膜通透性增加，这与磷脂组成的改变相对应。通过液相色谱/电喷雾电离质谱联用主成分分析，分析了鼓泡塔培养和摇瓶培养之间的磷脂差异。结果发现，磷脂酸（PA）是潜在生物标志物的主要分子种类。发现在鼓泡塔中培养96小时后，PA的含量增加，而磷脂酰胆碱（PC）和磷脂酰肌醇（PI）减少，这与在鼓泡塔中培养96小时后膜通透性的明显增加相对应。鼓泡塔喷嘴尺寸的减小导致磷脂的差异更加显著。这些结果表明，PA的积累在流体动力剪切应力诱导的膜降解中起重要作用。
