嗜热栖热菌属细菌之间的细胞壁结构差异

Cell wall structural divergence among Thermus spp.

作者信息

Quintela J C, Zöllner P, García-del Portillo F, Allmaier G, de Pedro M A

机构信息

Centro de Biologia Molecular "Severo Ochoa'', Consejo Superior de Investigaciones Cientificas-Universidad Autónoma de Madrid, Facultad de Ciencias, Campus de Cantoblanco, 28049 Madrid, Spain.

出版信息

FEMS Microbiol Lett. 1999 Mar 15;172(2):223-9. doi: 10.1111/j.1574-6968.1999.tb13472.x.

DOI:10.1111/j.1574-6968.1999.tb13472.x

PMID:19606554

Abstract

The fine structure of sacculi from Thermus thermophilus HB27, T. aquaticus YT-1 and Thermus ATCC27737 has been worked out by HPLC analysis and mass spectrometry techniques. The three microorganisms have a murein composition of the rare A3beta chemotype, but showed substantial differences in muropeptide composition. Phenylacetylated muropeptides,previously described in T. thermophilus HB8, were detected exclusively in T. thermophilus HB27. Murein from T. aquaticusYT-1 was devoid of D-Ala-D-Ala terminated muropeptides, which were, in contrast, abundant in T. thermophilus HB27 and Thermus ATCC27737. The significance of these findings is discussed.

摘要

通过高效液相色谱分析和质谱技术，已确定嗜热栖热菌HB27、嗜热水生栖热菌YT-1和嗜热栖热菌ATCC27737的囊泡精细结构。这三种微生物的胞壁质组成属于罕见的A3β化学型，但在胞壁肽组成上存在显著差异。先前在嗜热栖热菌HB8中描述的苯乙酰化胞壁肽仅在嗜热栖热菌HB27中检测到。嗜热水生栖热菌YT-1的胞壁质不含D-Ala-D-Ala末端的胞壁肽，相比之下，这些肽在嗜热栖热菌HB27和嗜热栖热菌ATCC27737中含量丰富。讨论了这些发现的意义。