嗜酸嗜热栖热菌葡萄糖脱氢酶的结晶及初步晶体学研究

Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum.

作者信息

Bright J R, Mackness R, Danson M J, Hough D W, Taylor G L, Towner P, Byrom D

机构信息

Department of Biochemistry, University of Bath Claverton Down, U.K.

出版信息

J Mol Biol. 1991 Nov 20;222(2):143-4. doi: 10.1016/0022-2836(91)90200-p.

DOI:10.1016/0022-2836(91)90200-p

PMID:1960718

Abstract

Single crystals of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum were obtained using the hanging-drop vapour diffusion method and polyethylene glycol as a precipitant in the presence of NADP+ at pH 5.4. The crystals belong to the hexagonal space group P6122 or P6522, with unit cell dimensions a = b = 121.9 angstrom, c = 229.6 angstrom and with two molecules in the asymmetric unit.

摘要

采用悬滴气相扩散法，以聚乙二醇作为沉淀剂，在pH 5.4且存在NADP⁺的条件下，获得了嗜热嗜酸栖热菌葡萄糖脱氢酶的单晶。这些晶体属于六方空间群P6₁22或P6₅22，晶胞参数a = b = 121.9埃，c = 229.6埃，不对称单元中有两个分子。