Lehwess-Litzmann Anja, Neumann Piotr, Golbik Ralph, Parthier Christoph, Tittmann Kai
Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August-Universität Göttingen, Ernst-Caspari-Haus, Justus-von-Liebig-Weg 11, D-37077 Göttingen, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):584-6. doi: 10.1107/S1744309111009274. Epub 2011 Apr 27.
The metabolic enzyme transaldolase from Thermoplasma acidophilum was recombinantly expressed in Escherichia coli and could be crystallized in two polymorphic forms. Crystals were grown by the hanging-drop vapour-diffusion method using PEG 6000 as precipitant. Native data sets for crystal forms 1 and 2 were collected in-house to resolutions of 3.0 and 2.7 Å, respectively. Crystal form 1 belonged to the orthorhombic space group C222(1) with five monomers per asymmetric unit and crystal form 2 belonged to the monoclinic space group P2(1) with ten monomers per asymmetric unit.
嗜热栖热菌的代谢酶转醛醇酶在大肠杆菌中进行了重组表达,并能以两种多晶型形式结晶。使用聚乙二醇6000作为沉淀剂,通过悬滴气相扩散法生长晶体。内部收集了晶型1和晶型2的天然数据集,分辨率分别为3.0 Å和2.7 Å。晶型1属于正交晶系空间群C222(1),每个不对称单元有五个单体,晶型2属于单斜晶系空间群P2(1),每个不对称单元有十个单体。