嗜酸热原体转醛醇酶的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum.
作者信息
Lehwess-Litzmann Anja, Neumann Piotr, Golbik Ralph, Parthier Christoph, Tittmann Kai
机构信息
Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August-Universität Göttingen, Ernst-Caspari-Haus, Justus-von-Liebig-Weg 11, D-37077 Göttingen, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):584-6. doi: 10.1107/S1744309111009274. Epub 2011 Apr 27.
Abstract
The metabolic enzyme transaldolase from Thermoplasma acidophilum was recombinantly expressed in Escherichia coli and could be crystallized in two polymorphic forms. Crystals were grown by the hanging-drop vapour-diffusion method using PEG 6000 as precipitant. Native data sets for crystal forms 1 and 2 were collected in-house to resolutions of 3.0 and 2.7 Å, respectively. Crystal form 1 belonged to the orthorhombic space group C222(1) with five monomers per asymmetric unit and crystal form 2 belonged to the monoclinic space group P2(1) with ten monomers per asymmetric unit.
摘要
嗜热栖热菌的代谢酶转醛醇酶在大肠杆菌中进行了重组表达，并能以两种多晶型形式结晶。使用聚乙二醇6000作为沉淀剂，通过悬滴气相扩散法生长晶体。内部收集了晶型1和晶型2的天然数据集，分辨率分别为3.0 Å和2.7 Å。晶型1属于正交晶系空间群C222(1)，每个不对称单元有五个单体，晶型2属于单斜晶系空间群P2(1)，每个不对称单元有十个单体。
相似文献
1
Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum.嗜酸热原体转醛醇酶的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):584-6. doi: 10.1107/S1744309111009274. Epub 2011 Apr 27.
2
Crystallization and preliminary crystallographic study of citrate synthase from the thermophilic Archaeon Thermoplasma acidophilum.嗜热古菌嗜酸热原体柠檬酸合酶的结晶及初步晶体学研究
J Mol Biol. 1993 Jul 5;232(1):308-9. doi: 10.1006/jmbi.1993.1386.
3
Crystallization and preliminary X-ray diffraction analysis of the metalloregulatory protein DtxR from Thermoplasma acidophilum.嗜热栖热放线菌金属调节蛋白DtxR的结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):172-4. doi: 10.1107/S1744309111051700. Epub 2012 Jan 26.
4
Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum.嗜酸嗜热栖热菌葡萄糖脱氢酶的结晶及初步晶体学研究
J Mol Biol. 1991 Nov 20;222(2):143-4. doi: 10.1016/0022-2836(91)90200-p.
5
6
Preliminary X-ray crystallographic study of the proteasome from Thermoplasma acidophilum.嗜热栖热菌蛋白酶体的初步X射线晶体学研究。
J Mol Biol. 1993 Dec 5;234(3):881-4. doi: 10.1006/jmbi.1993.1633.
7
Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum.嗜热栖热菌甘油醛脱氢酶的结晶行为
Acta Crystallogr F Struct Biol Commun. 2015 Dec;71(Pt 12):1475-80. doi: 10.1107/S2053230X15020270. Epub 2015 Nov 18.
8
Crystallization and preliminary crystallographic analysis of NAD+-preferring aldohexose dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum.嗜热嗜酸古菌嗜热栖热菌中NAD⁺偏好型醛己糖脱氢酶的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt 6):586-9. doi: 10.1107/S1744309106017362. Epub 2006 May 31.
9
Overexpression, crystallization and preliminary X-ray crystallographic analysis of a putative transposase from Thermoplasma acidophilum encoded by the Ta0474 gene.嗜热栖热菌Ta0474基因编码的一种假定转座酶的过表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1147-9. doi: 10.1107/S174430910604245X. Epub 2006 Oct 20.
10
Expression, crystallization and preliminary X-ray crystallographic analyses of two N-terminal acetyltransferase-related proteins from Thermoplasma acidophilum.嗜酸热原体中两种N端乙酰转移酶相关蛋白的表达、结晶及初步X射线晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1127-30. doi: 10.1107/S1744309106040267. Epub 2006 Oct 20.
引用本文的文献
1
Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism.扭曲的席夫碱中间体和底物位置改变了转醛醇酶的机制。
Nat Chem Biol. 2011 Aug 21;7(10):678-84. doi: 10.1038/nchembio.633.
本文引用的文献
1
XDS.XDS.（这个词如果没有更多背景信息，很难准确翻译出更有意义的内容，直接保留原文是一种处理方式，或者音译为“克斯达斯”之类，但感觉都不太符合常规翻译场景，你可以补充更多关于这个词的信息以便我更准确翻译 ）
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. doi: 10.1107/S0907444909047337. Epub 2010 Jan 22.
2
Transaldolase: from biochemistry to human disease.转醛醇酶：从生物化学到人类疾病
Int J Biochem Cell Biol. 2009 Jul;41(7):1482-94. doi: 10.1016/j.biocel.2009.02.001. Epub 2009 Feb 11.
3
Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.酶促硫胺素催化中的应变和接近攻击构象：细菌转酮醇酶与供体酮糖5-磷酸木酮糖和6-磷酸果糖形成共价复合物，以及与受体醛糖5-磷酸核糖形成非共价复合物的X射线晶体学快照。
Biochemistry. 2007 Oct 30;46(43):12037-52. doi: 10.1021/bi700844m. Epub 2007 Oct 3.
4
Crystallization and preliminary X-ray crystallographic analysis of recombinant transaldolase B from Eschericha coli.来自大肠杆菌的重组转醛醇酶B的结晶及初步X射线晶体学分析。
Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):192-3. doi: 10.1107/S0907444995010365.
5
Identification of catalytically important residues in the active site of Escherichia coli transaldolase.大肠杆菌转醛醇酶活性位点中具有催化重要性的残基的鉴定。
Eur J Biochem. 2001 Apr;268(8):2408-15. doi: 10.1046/j.1432-1327.2001.02128.x.
6
The three-dimensional structure of human transaldolase.人转醛醇酶的三维结构。
FEBS Lett. 2000 Jun 23;475(3):205-8. doi: 10.1016/s0014-5793(00)01658-6.
7
Crystal structure of the reduced Schiff-base intermediate complex of transaldolase B from Escherichia coli: mechanistic implications for class I aldolases.大肠杆菌转醛醇酶B还原席夫碱中间体复合物的晶体结构：I类醛缩酶的机制意义
Protein Sci. 1997 Jan;6(1):119-24. doi: 10.1002/pro.5560060113.
8
Crystal structure of transaldolase B from Escherichia coli suggests a circular permutation of the alpha/beta barrel within the class I aldolase family.来自大肠杆菌的转醛醇酶B的晶体结构表明I类醛缩酶家族中α/β桶状结构存在环状排列。
Structure. 1996 Jun 15;4(6):715-24. doi: 10.1016/s0969-2126(96)00077-9.
Zotero 插件