Baniahmad C, Muller M, Altschmied J, Renkawitz R
Max-Planck-Institut für Biochemie, Martinsried, Germany.
J Mol Biol. 1991 Nov 20;222(2):155-65. doi: 10.1016/0022-2836(91)90202-h.
Steroid induction of responsive genes functions through the synergistic activity of steroid receptor binding sequences with adjacent binding sites either for other transcription factors or for further steroid receptors. Analysis of the human glucocorticoid receptor revealed that the DNA-binding domain of the receptor is sufficient to mediate co-operative binding to adjacent receptor binding sites. This is a novel feature of the domain in addition to its DNA-binding, trans-activating and trans-repressing properties. Chimaeric proteins containing the N- or C-terminal receptor halves fused to the GAL4 DNA-binding domain do not co-operate in DNA-binding, however they do functionally synergize. Thus, at least two mechanisms contribute to the synergism of the human glucocorticoid receptor bound to two adjacent receptor binding sites.
类固醇对反应性基因的诱导作用是通过类固醇受体结合序列与相邻的其他转录因子或其他类固醇受体结合位点的协同活性来实现的。对人糖皮质激素受体的分析表明，该受体的DNA结合结构域足以介导与相邻受体结合位点的协同结合。这是该结构域除了其DNA结合、反式激活和反式抑制特性之外的一个新特征。含有与GAL4 DNA结合结构域融合的N端或C端受体半段的嵌合蛋白在DNA结合方面不具有协同作用，但它们在功能上具有协同作用。因此，至少有两种机制促成了与人糖皮质激素受体结合到两个相邻受体结合位点时的协同作用。
