Chang C, Gralla J D
Department of Chemistry and Biochemistry, University of California, Los Angeles 90024-1569.
Mol Cell Biol. 1994 Aug;14(8):5175-81. doi: 10.1128/mcb.14.8.5175-5181.1994.
The role of chromatin in mounting a synergistic transcriptional response to GAL4-VP16 was investigated. Strong synergy was observed when chromatin templates were used in vitro. The synergy was severely reduced when naked DNA templates were transcribed. In vivo synergy was strong when nonreplicating templates were used. However, the use of replicating templates, which involved transient disruptions of chromatin, led to strong reductions in synergy. In both of these low-synergy responses, transcription levels were high. We infer that strong synergy has a requirement for chromatin that may be understood in terms of the competition between multiple activator molecules and histone cores for promoter DNA.
研究了染色质在对GAL4-VP16产生协同转录反应中的作用。当在体外使用染色质模板时，观察到强烈的协同作用。当转录裸DNA模板时，协同作用严重降低。当使用非复制模板时，体内协同作用很强。然而，使用涉及染色质瞬时破坏的复制模板会导致协同作用大幅降低。在这两种低协同反应中，转录水平都很高。我们推断，强烈的协同作用需要染色质，这可以从多个激活分子与组蛋白核心竞争启动子DNA的角度来理解。
