Sadkowski T, Jank M, Zwierzchowski L, Oprzadek J, Motyl T
Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland.
J Physiol Pharmacol. 2009 May;60 Suppl 1:15-28.
In the present study cDNA microarray (18263 probes) were used for analysis of bovine skeletal muscle (m.semitendinosus) transcriptome in 12-month-old bulls of four cattle breeds: Holstein-Friesian (HF), Limousine (LIM), Hereford (HER) and Polish Red (PR), aiming to identify the genes, which expression is common for beef breed bulls. The number of transcripts significantly different from HF bulls muscle amounted to 393, 462 and 638 for LIM, HER and PR, respectively. As a result of this study the transcriptomic index was proposed, being the set of 48 genes expressed similarly in beef breed bulls in comparison to HF bulls. Classification of genes according to molecular function of their protein products has shown the highest number of genes encoding proteins involved in nucleic acid binding (10), regulatory proteins (6), kinases (4) and signaling molecules (3). Classification according to biological processes revealed the highest number of genes involved in protein metabolism i modification (14), signal transduction (5), cell cycle (4), intracellular protein traffic (4), nucleoside, nucleotide and nucleic acid metabolism (4), apoptosis (3), cell structure and motility (3), and cellular transport (3). Since the role of the most genes included to the transcriptomic index has not been described yet in bovine skeletal muscle, obtained results may be very useful in revealing new candidate genes to search a new criteria of animal selection in beef production.
在本研究中，利用cDNA微阵列（18263个探针）对四个牛品种（荷斯坦-弗里生牛（HF）、利木赞牛（LIM）、海福特牛（HER）和波兰红牛（PR））12月龄公牛的半腱肌转录组进行分析，旨在鉴定在肉牛品种公牛中表达普遍的基因。与HF公牛肌肉相比，LIM、HER和PR公牛中差异显著的转录本数量分别为393、462和638个。作为本研究的结果，提出了转录组指数，即与HF公牛相比，在肉牛品种公牛中以相似方式表达的48个基因的集合。根据其蛋白质产物的分子功能对基因进行分类，结果显示编码参与核酸结合的蛋白质的基因数量最多（10个），其次是调节蛋白（6个）、激酶（4个）和信号分子（3个）。根据生物学过程进行分类，发现参与蛋白质代谢与修饰的基因数量最多（14个），其次是信号转导（5个）、细胞周期（4个）、细胞内蛋白质运输（4个）、核苷、核苷酸和核酸代谢（4个）、细胞凋亡（3个）、细胞结构与运动（3个）以及细胞运输（3个）。由于转录组指数中包含的大多数基因在牛骨骼肌中的作用尚未被描述，因此获得的结果可能对揭示新的候选基因非常有用，从而有助于寻找肉牛生产中动物选择的新标准。
